Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of a recombinant GST fused enzyme | Phaeosphaeria sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl-piperidine-1-carboxylate | AMO-1618, a quaternary ammonium salt, leads to total enzyme inhibition at 0.001 mM | Phaeosphaeria sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
calculated from amino acid sequence | Phaeosphaeria sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeosphaeria sp. | O13284 | from mycelia | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis | Phaeosphaeria sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | bifunctional enzyme | Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Phaeosphaeria sp. | copalyl diphosphate | - |
? |