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Literature summary for 5.5.1.13 extracted from

  • Kawaide, H.; Imai, R.; Sassa, T.; Kamiya, Y.
    ent-Kaurene synthetase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis (1997), J. Biol. Chem., 272, 21706-21712.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of a recombinant GST fused enzyme Phaeosphaeria sp.

Inhibitors

Inhibitors Comment Organism Structure
2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl-piperidine-1-carboxylate AMO-1618, a quaternary ammonium salt, leads to total enzyme inhibition at 0.001 mM Phaeosphaeria sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
calculated from amino acid sequence Phaeosphaeria sp.

Organism

Organism UniProt Comment Textmining
Phaeosphaeria sp. O13284 from mycelia
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis Phaeosphaeria sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
copalyl diphosphate bifunctional enzyme Phaeosphaeria sp. ent-kaurene + diphosphate
-
?
geranylgeranyl diphosphate
-
Phaeosphaeria sp. copalyl diphosphate
-
?