Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.5.1.12 extracted from

  • Gnanasekaran, T.; Vavitsas, K.; Andersen-Ranberg, J.; Nielsen, A.Z.; Olsen, C.E.; Hamberger, B.; Jensen, P.E.
    Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme (2015), J. Biol. Eng., 9, 24 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of Picea abies isopimara-7,15-diene synthase (diTPS-ISO) in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection method, coexpression with a multifunctional cytochrome P450 (CYP720B4) from Picea sitchensis, subcloning in Escherichia coli strain NEB10beta Picea abies

Protein Variants

Protein Variants Comment Organism
additional information transient heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana. Expressing DXS and GGPPs from Plectranthus barbatus in Nicotiana benthamiana are efficient in boosting diterpenoid levels compared to coexpression of the Nicotiana endogenous DXS and GGPPs genes, which have no effect on boosting diterpenoid level. Pigment analysis of infiltrated leaves, overview Picea abies

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Picea abies 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Picea abies

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Picea abies
-
(+)-copalyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Picea abies Q675L5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Picea abies
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
Picea abies (+)-copalyl diphosphate
-
?
additional information the bifunctional diterpene synthase isopimara-7, 15-diene synthase (diTPS-ISO) catalyzes the cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate and the formation of isopimara-7,15-diene from (+)-copalyl diphosphate (cf. EC 4.2.3.44). Compound identification and quantification by GC-MS analysis Picea abies ?
-
?

Synonyms

Synonyms Comment Organism
diTPS-ISO
-
Picea abies
More cf. EC 4.2.3.44 Picea abies

General Information

General Information Comment Organism
metabolism the bifunctional diterpene synthase, isopimara-7, 15-diene synthase (diTPS-ISO) and a multifunctional cytochrome P450, CYP720B4 perform the biosynthesis of isopimaric acid. The diterpene synthase converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene) via formation of (+)-copalyl diphosphate. In three subsequent oxygenation reactions, the endoplasmic reticulum-associated enzyme CYP720B4 catalyzes the conversion of isopimaradiene into isopimaric acid, pathway overview Picea abies
physiological function the chloroplastidic bifunctional diterpene synthase, isopimara-7,15-diene synthase (diTPS-ISO) from Picea abies and a cytosolic multifunctional cytochrome P450 (CYP720B4, UniProt ID E5FA70) from Picea sitchensis, which is associated to the endoplasmic reticulum. The diterpene synthase diTPS-ISO converts geranylgeranyl diphosphate (GGPP) to the tricyclic diterpene isopimara-7,15-diene (isopimaradiene) Picea abies