Protein Variants | Comment | Organism |
---|---|---|
H315N/T316V | site-directed mutagenesis, the double mutant produces terpentedienyl diphosphate, which is derived from complete substituent rearrangement of syn rather than normal labdaenyl intermediate. Alteration of the residues that normally serve as the catalytic base can impact stereocontrol | Salvia miltiorrhiza |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Salvia miltiorrhiza | - |
(+)-copalyl diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia miltiorrhiza | B8PQ84 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Salvia miltiorrhiza | (+)-copalyl diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CPP synthase | - |
Salvia miltiorrhiza |
SmCPS | - |
Salvia miltiorrhiza |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a class II diterpene cyclase | Salvia miltiorrhiza |
malfunction | changing two adjacent residues that seem to serve as the catalytic base in the normal CPP synthase from Salvia miltiorrhiza (SmCPS) to the residues found in the closely related perigrinol diphosphate synthase from Marrubium vulgare (MvPPS), which produces a partially rearranged and hydroxylated product derived from the distinct syn stereoisomer of labdaenyl intermediate, alters the product outcome, cf. EC 5.5.1.14. Specifically, the relevant SmCPS:H315N/T316V double mutant produces terpentedienyl diphosphate, which is derived from complete substituent rearrangement of syn rather than normal labdaenyl intermediate. Alteration of the residues that normally serve as the catalytic base can impact stereocontrol | Salvia miltiorrhiza |
additional information | class II diterpene cyclases (DTCs) utilize a general acid-base catalyzed cyclo-isomerization reaction to produce various stereoisomers of the eponymous labdaenyl carbocation intermediate, which can then undergo rearrangement and/or the addition of water prior to terminating deprotonation. Identification of the pair of residues that cooperatively serves as the catalytic base in the DTCs producing ent-copalyl diphosphate (CPP) required for gibberellin phytohormone biosynthesis in all vascular plants, overview | Salvia miltiorrhiza |