Cloned (Comment) | Organism |
---|---|
gene SmCPS1, phylogenetic analysis, a 289-bp gene-specific sequence including the 3' UTR of SmCPS1 is amplified by PCR using cDNA as a template and then cloned into the pK7GWIWG (II) binary, then introduced into Agrobacterium tumefaciens strain EHA105 by electroporation for ransformation of leaf explants from Salvia miltiorrhiza bh2-7 plants, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3) | Salvia miltiorrhiza f. alba |
gene SmCPS2, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3) | Salvia miltiorrhiza f. alba |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of SmCPS1 by RNAi | Salvia miltiorrhiza f. alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Salvia miltiorrhiza f. alba | - |
(+)-copalyl diphosphate | - |
r | |
geranylgeranyl diphosphate | Salvia miltiorrhiza f. alba bh2-7 | - |
(+)-copalyl diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia miltiorrhiza f. alba | A0A0A7AN31 | - |
- |
Salvia miltiorrhiza f. alba | A0A0A7ANT1 | - |
- |
Salvia miltiorrhiza f. alba bh2-7 | A0A0A7AN31 | - |
- |
Salvia miltiorrhiza f. alba bh2-7 | A0A0A7ANT1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strains Tuner (DE3) or Origami B (DE3) | Salvia miltiorrhiza f. alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Salvia miltiorrhiza f. alba | (+)-copalyl diphosphate | - |
r | |
geranylgeranyl diphosphate | - |
Salvia miltiorrhiza f. alba bh2-7 | (+)-copalyl diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CPS1 | - |
Salvia miltiorrhiza f. alba |
CPS2 | - |
Salvia miltiorrhiza f. alba |
SmCPS1 | - |
Salvia miltiorrhiza f. alba |
SmCPS2 | - |
Salvia miltiorrhiza f. alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Salvia miltiorrhiza f. alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salvia miltiorrhiza f. alba |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the diterpene synthase family | Salvia miltiorrhiza f. alba |
additional information | metabolomic profiling, and homology modeling and molecular docking | Salvia miltiorrhiza f. alba |