Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.5.1.12 extracted from

  • Brueckner, K.; Bozic, D.; Manzano, D.; Papaefthimiou, D.; Pateraki, I.; Scheler, U.; Ferrer, A.; de Vos, R.C.; Kanellis, A.K.; Tissier, A.
    Characterization of two genes for the biosynthesis of abietane-type diterpenes in rosemary (Rosmarinus officinalis) glandular trichomes (2014), Phytochemistry, 101, 52-64 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CPS1, phylogenetic analysis and tree, functional recombinant expression in Saccharomyces cerevisiae strain AM113, with co-overexpressing of GGDPS1 from Cistus creticus, and Nicotiana benthamiana, heterologous expression of the RoCPS1 protein in Escherichia coli fails Salvia rosmarinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Salvia rosmarinus
-
(+)-copalyl diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Salvia rosmarinus W8QQT6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
glandular trichome
-
Salvia rosmarinus
-
leaf young and old Salvia rosmarinus
-
additional information quantitative RT-PCR expression analysis on whole leaves shows that RoCPS1 is preferentially expressed in in trichomes from young leaves Salvia rosmarinus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
Salvia rosmarinus (+)-copalyl diphosphate
-
r

Synonyms

Synonyms Comment Organism
CPS1
-
Salvia rosmarinus
RoCPS1
-
Salvia rosmarinus

General Information

General Information Comment Organism
metabolism several stereoisomers of copalyl diphosphate (CDP) are found, among which the most common is ent-CDP, the precursor of gibberellins. Other isomers are syn-CDP and normal-CDP, the latter being utilized for the biosynthesis of diterpene resin acids in Gymnosperms and abietane-type tanshinones in Salvia miltiorrhiza. The second step of the cyclization is initiated through the formation of a carbocation upon ionization of the diphosphate linkage of CDP. This is catalyzed by class I terpene synthases which can transform CDP into a variety of polycyclic diterpenoids. By analogy to other labdane-type diterpenoids, the biosynthesis of the diterpene precursor of the carnosic acid pathway in rosemary proceeds in two sequential steps catalyzed by two distinct enzymes. The first is a class II TPS enzyme, CPS1, that yields normal CDP, which then is converted to miltiradiene by a KSL enzyme, miltiradiene synthase, EC 4.2.3.131, which apears in two isoforms, RoKSL1 and RoKSL2, in Rosmarinus officinalis. Hypothetical biosynthetic pathway of carnosic acid and carnosol produced in Rosmarinus officinalis, overview Salvia rosmarinus