Cloned (Comment) | Organism |
---|---|
gene CPS1, phylogenetic analysis and tree, functional recombinant expression in Saccharomyces cerevisiae strain AM113, with co-overexpressing of GGDPS1 from Cistus creticus, and Nicotiana benthamiana, heterologous expression of the RoCPS1 protein in Escherichia coli fails | Salvia rosmarinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Salvia rosmarinus | - |
(+)-copalyl diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia rosmarinus | W8QQT6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
glandular trichome | - |
Salvia rosmarinus | - |
leaf | young and old | Salvia rosmarinus | - |
additional information | quantitative RT-PCR expression analysis on whole leaves shows that RoCPS1 is preferentially expressed in in trichomes from young leaves | Salvia rosmarinus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Salvia rosmarinus | (+)-copalyl diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CPS1 | - |
Salvia rosmarinus |
RoCPS1 | - |
Salvia rosmarinus |
General Information | Comment | Organism |
---|---|---|
metabolism | several stereoisomers of copalyl diphosphate (CDP) are found, among which the most common is ent-CDP, the precursor of gibberellins. Other isomers are syn-CDP and normal-CDP, the latter being utilized for the biosynthesis of diterpene resin acids in Gymnosperms and abietane-type tanshinones in Salvia miltiorrhiza. The second step of the cyclization is initiated through the formation of a carbocation upon ionization of the diphosphate linkage of CDP. This is catalyzed by class I terpene synthases which can transform CDP into a variety of polycyclic diterpenoids. By analogy to other labdane-type diterpenoids, the biosynthesis of the diterpene precursor of the carnosic acid pathway in rosemary proceeds in two sequential steps catalyzed by two distinct enzymes. The first is a class II TPS enzyme, CPS1, that yields normal CDP, which then is converted to miltiradiene by a KSL enzyme, miltiradiene synthase, EC 4.2.3.131, which apears in two isoforms, RoKSL1 and RoKSL2, in Rosmarinus officinalis. Hypothetical biosynthetic pathway of carnosic acid and carnosol produced in Rosmarinus officinalis, overview | Salvia rosmarinus |