Cloned (Comment) | Organism |
---|---|
gene catB | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
2-methyl-cis,cis-muconate | 24°C, pH 8.0 | Arthrobacter sp. | |
0.046 | - |
3-chloro-cis,cis-muconate | 24°C, pH 8.0 | Arthrobacter sp. | |
0.075 | - |
3-methyl-cis,cis-muconate | 24°C, pH 8.0 | Arthrobacter sp. | |
0.15 | - |
cis,cis-muconate | 24°C, pH 8.0 | Arthrobacter sp. | |
0.67 | - |
3-ethyl-cis,cis-muconate | 24°C, pH 8.0 | Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 56% of the activity with Mn2+ | Arthrobacter sp. | |
Mn2+ | required, 25% residual activity without addition of Mn2+ | Arthrobacter sp. | |
additional information | Co2+ may not replace for Mn2+ | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40926 | - |
6 * 43000, SDS-PAGE, 6 * 40926, calculated | Arthrobacter sp. |
43000 | - |
6 * 43000, SDS-PAGE, 6 * 40926, calculated | Arthrobacter sp. |
280000 | - |
gel filtration | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. BA-5-17 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41 | - |
24°C, pH 8.0 | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-cis,cis-muconate | - |
Arthrobacter sp. | 2-methylmuconolactone | - |
? | |
3-Chloro-cis,cis-muconate | - |
Arthrobacter sp. | 5-Chloromuconolactone | - |
? | |
3-Chloro-cis,cis-muconate | - |
Arthrobacter sp. BA-5-17 | 5-Chloromuconolactone | - |
? | |
3-ethyl-cis,cis-muconate | - |
Arthrobacter sp. | 4-ethylmuconolactone | - |
? | |
3-ethyl-cis,cis-muconate | - |
Arthrobacter sp. BA-5-17 | 4-ethylmuconolactone | - |
? | |
3-methyl-cis,cis-muconate | - |
Arthrobacter sp. | 4-methylmuconolactone | - |
? | |
3-methyl-cis,cis-muconate | - |
Arthrobacter sp. BA-5-17 | 4-methylmuconolactone | - |
? | |
cis,cis-Muconate | - |
Arthrobacter sp. | (+)-Muconolactone | - |
? | |
cis,cis-Muconate | - |
Arthrobacter sp. BA-5-17 | (+)-Muconolactone | - |
? | |
additional information | no substrate: 2-chloro-cis,cis-muconate, 2-fluoro-cis,cis-muconate | Arthrobacter sp. | ? | - |
? | |
additional information | no substrate: 2-chloro-cis,cis-muconate, 2-fluoro-cis,cis-muconate | Arthrobacter sp. BA-5-17 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 43000, SDS-PAGE, 6 * 40926, calculated | Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10 min, more than 90% residual activity | Arthrobacter sp. |
70 | - |
10 min, complete loss of activity | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | more than 70% of maximum activity within this range | Arthrobacter sp. |