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Literature summary for 5.5.1.1 extracted from
- Structural basis for the activity of two muconate cycloisomerase variants toward substituted muconates (1999), Proteins Struct. Funct. Genet., 34, 125-136.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| muatnt enzyme F329I and I54V | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F329I | the ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.5fold increase with 2-chloro-cis,cis-muconate, 3.4fold increased with 3-chloro-cis,cis-muconate, 3.16fold increase with 2,4-dichloro-cis,cis-muconate, 1.7fold decrease with 3-fluoro-cis,cis-muconate, 1.55fold increase with 2-methyl-cis,cis-muconate and 5.8fold increase with 3-methyl-cis,cis-muconate | Pseudomonas putida |
| I54V | ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical with 2-chloro-cis,cis-muconate, 22fold increase with 3-chloro-cis,cis-muconate, 1.6fold decrease with 2,4-dichloro-cis,cis-muconate, 5.75fold decrease with 3-fluoro-cis,cis-muconate, 6fold decrease with 2-methyl-cis,cis-muconate and 1.3fold increase with 3-methyl-cis,cis-muconate | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | P08310 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dichloro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? |  |
| 2-chloro-cis,cis-muconate | - |
Pseudomonas putida | 5-chloromuconolactone + 2-chloromuconolactone | - |
? | |
| 2-Methyl-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
| 3-chloro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
| 3-Fluoro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
| 3-Methyl-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
| cis,cis-muconate | - |
Pseudomonas putida | muconolactone | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCIase | - |
Pseudomonas putida |
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Release 2023.1 (January 2023)
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