Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 1.0 mM, complete inhibition of MC I and MC II | Frateuria sp. | |
EDTA | 1.0 mM, complete inhibition of MC I and MC II | Frateuria sp. | |
HgCl2 | 0.5 mM, complete inhibition of enzyme form MC I and MC II | Frateuria sp. | |
PCMB | - |
Frateuria sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 6% of the activity with Mn2+, enzyme form MC II | Frateuria sp. | |
Fe2+ | 0.4% of the activity with Mn2+, enzyme form MC II | Frateuria sp. | |
Mg2+ | 28% of the activity with Mn2+, enzyme form MC I. 17% of the activity with Mn2+, enzyme form MC II | Frateuria sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Frateuria sp. |
400000 | - |
gel filtration | Frateuria sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-muconate | Frateuria sp. | different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 | muconolactone | - |
? | |
cis,cis-muconate | Frateuria sp. ANA-18 | different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 | muconolactone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Frateuria sp. | - |
ANA-18 | - |
Frateuria sp. ANA-18 | - |
ANA-18 | - |
Purification (Comment) | Organism |
---|---|
- |
Frateuria sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Frateuria sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-muconate | - |
Frateuria sp. | muconolactone | - |
? | |
cis,cis-muconate | different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 | Frateuria sp. | muconolactone | - |
? | |
cis,cis-muconate | - |
Frateuria sp. ANA-18 | muconolactone | - |
? | |
cis,cis-muconate | different inducible production of MC I and MC II suggest that two catechol branches involved in the beta-ketoadipate pathway function in Frateuria sp. ANA-18 | Frateuria sp. ANA-18 | muconolactone | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Frateuria sp. |
Synonyms | Comment | Organism |
---|---|---|
MC II | - |
Frateuria sp. |
MC O | - |
Frateuria sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, 65% loss of activity of enzyme form MC I | Frateuria sp. |
75 | - |
10 min, 19% loss of activity of enzyme form MC II | Frateuria sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
enzyme form MC I and MC II | Frateuria sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | enzyme form MCI shows more than 80% of maximal activity in the range pH 5.5-7.5 | Frateuria sp. |
6 | 7 | enzyme form MCII shows more than 80% of maximal activity in the range pH 6.0-7.0 | Frateuria sp. |