BRENDA - Enzyme Database
show all sequences of 5.4.99.B4

Archaeal proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA

Kamalampeta, R.; Kothe, U.; Sci. Rep. 2, 663 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Gar1
the activity of Cbf5 is enhanced by protein Gar1 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction)
Pyrococcus furiosus
Nop10
the activity of Cbf5 is enhanced by protein Nop10 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction)
Pyrococcus furiosus
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli Rosetta 2(DE3) cells
Pyrococcus furiosus
Organism
Organism
UniProt
Commentary
Textmining
Pyrococcus furiosus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni2+ Sepharose column chromatography and Superdex 75 gel filtration
Pyrococcus furiosus
Synonyms
Synonyms
Commentary
Organism
Cbf5
-
Pyrococcus furiosus
pseudouridine synthase
-
Pyrococcus furiosus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Gar1
the activity of Cbf5 is enhanced by protein Gar1 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction)
Pyrococcus furiosus
Nop10
the activity of Cbf5 is enhanced by protein Nop10 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction)
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta 2(DE3) cells
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+ Sepharose column chromatography and Superdex 75 gel filtration
Pyrococcus furiosus
General Information
General Information
Commentary
Organism
physiological function
the enzyme usually acts in a guide RNA-dependent manner as part of H/ACA small ribonucleoproteins, however, archaeal Cbf5 can also act independently of guide RNA in modifying uridine 55 in tRNA
Pyrococcus furiosus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme usually acts in a guide RNA-dependent manner as part of H/ACA small ribonucleoproteins, however, archaeal Cbf5 can also act independently of guide RNA in modifying uridine 55 in tRNA
Pyrococcus furiosus
Other publictions for EC 5.4.99.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730948
Kamalampeta
Archaeal proteins Nop10 and Ga ...
Pyrococcus furiosus
Sci. Rep.
2
663
2012
2
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714379
Kipper
Pseudouridylation of 23S rRNA ...
Escherichia coli
Biochimie
93
834-844
2011
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715376
O’Connor
Inactivation of the RluD pseud ...
Escherichia coli, Salmonella enterica
J. Bacteriol.
193
154-162
2011
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2
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716902
Blaby
Pseudouridine formation in arc ...
Haloferax volcanii, Haloferax volcanii DSM 3757, Haloferax volcanii H26
RNA
17
1367-1380
2011
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715198
Sato
Genetic analyses of the antibi ...
Bifidobacterium bifidum, Bifidobacterium bifidum Yakult YIT 4001, no activity in Bifidobacterium bifidum strain Yakult YIT 4007
Int. J. Food Microbiol.
137
254-258
2010
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716899
Ero
Specificity and kinetics of 23 ...
Escherichia coli
RNA
16
2075-2084
2010
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