BRENDA - Enzyme Database show
show all sequences of 5.4.99.B4

Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii

Blaby, I.K.; Majumder, M.; Chatterjee, K.; Jana, S.; Grosjean, H.; de Crecy-Lagard, V.; Gupta, R.; RNA 17, 1367-1380 (2011)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Haloferax volcanii
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
?
-
-
-
additional information
Haloferax volcanii DSM 3757
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloferax volcanii
D4GTP8
-
-
Haloferax volcanii
-
-
-
Haloferax volcanii DSM 3757
D4GTP8
-
-
Haloferax volcanii H26
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine
Cbf5 is needed for pseudouridylation of the large subunit rRNA residues
716902
Haloferax volcanii
23S rRNA pseudouridine
-
-
-
?
23S rRNA uridine
Cbf5 is needed for pseudouridylation of the large subunit rRNA residues
716902
Haloferax volcanii H26
23S rRNA pseudouridine
-
-
-
?
additional information
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
716902
Haloferax volcanii
?
-
-
-
-
additional information
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
716902
Haloferax volcanii DSM 3757
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Haloferax volcanii
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
?
-
-
-
additional information
Haloferax volcanii DSM 3757
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine
Cbf5 is needed for pseudouridylation of the large subunit rRNA residues
716902
Haloferax volcanii
23S rRNA pseudouridine
-
-
-
?
23S rRNA uridine
Cbf5 is needed for pseudouridylation of the large subunit rRNA residues
716902
Haloferax volcanii H26
23S rRNA pseudouridine
-
-
-
?
additional information
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
716902
Haloferax volcanii
?
-
-
-
-
additional information
pseudouridylation of Haloferax volcanii 23S rRNA is Cbf5-mediated
716902
Haloferax volcanii DSM 3757
?
-
-
-
-
General Information
General Information
Commentary
Organism
malfunction
cbf5 deletion reduces the amounts of guide box H/ACA RNAs; deletion of cbf5 does not lead to any obvious phenotype, the Cbf5-deleted strain of Haloferax volcanii completely lacks pseudouridine at positions 1940, 1942, 2605, and 2591 of its 23S rRNA, and contains reduced steady-state levels of some box H/ACA RNAs
Haloferax volcanii
physiological function
the Cbf5 homolog (HVO 2493) is not essential in Haloferax volcanii. Cbf5 is not needed for most of the tRNA pseudouridine residues of Haloferax volcanii; the enzyme Cbf5 is responsible for making pseudouridine at multiple positions of the large subunit of rRNA
Haloferax volcanii
General Information (protein specific)
General Information
Commentary
Organism
malfunction
cbf5 deletion reduces the amounts of guide box H/ACA RNAs
Haloferax volcanii
malfunction
deletion of cbf5 does not lead to any obvious phenotype, the Cbf5-deleted strain of Haloferax volcanii completely lacks pseudouridine at positions 1940, 1942, 2605, and 2591 of its 23S rRNA, and contains reduced steady-state levels of some box H/ACA RNAs
Haloferax volcanii
physiological function
the enzyme Cbf5 is responsible for making pseudouridine at multiple positions of the large subunit of rRNA
Haloferax volcanii
physiological function
the Cbf5 homolog (HVO 2493) is not essential in Haloferax volcanii. Cbf5 is not needed for most of the tRNA pseudouridine residues of Haloferax volcanii
Haloferax volcanii
Other publictions for EC 5.4.99.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730948
Kamalampeta
Archaeal proteins Nop10 and Ga ...
Pyrococcus furiosus
Sci. Rep.
2
663
2012
2
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1
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1
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1
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1
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1
1
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714379
Kipper
Pseudouridylation of 23S rRNA ...
Escherichia coli
Biochimie
93
834-844
2011
-
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715376
OConnor
Inactivation of the RluD pseud ...
Escherichia coli, Salmonella enterica
J. Bacteriol.
193
154-162
2011
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2
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2
2
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716902
Blaby
Pseudouridine formation in arc ...
Haloferax volcanii, Haloferax volcanii DSM 3757, Haloferax volcanii H26
RNA
17
1367-1380
2011
-
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2
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13
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4
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4
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2
4
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715198
Sato
Genetic analyses of the antibi ...
Bifidobacterium bifidum, Bifidobacterium bifidum Yakult YIT 4001, no activity in Bifidobacterium bifidum strain Yakult YIT 4007
Int. J. Food Microbiol.
137
254-258
2010
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2
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3
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716899
Ero
Specificity and kinetics of 23 ...
Escherichia coli
RNA
16
2075-2084
2010
-
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1
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1
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1
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1
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3
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1
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1
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1
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1
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3
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1
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1
1