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show all sequences of 5.4.99.B4

Inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function in wild-type Escherichia coli and Salmonella enterica

O’Connor, M.; Gregory, S.T.; J. Bacteriol. 193, 154-162 (2011)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Salmonella enterica
-
serovar typhimurium
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine
pseudouridine synthase is responsible for the pseudouridine modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA
715376
Escherichia coli
23S rRNA pseudouridine
-
-
-
?
23S rRNA uridine
pseudouridine synthase is responsible for the pseudouridine modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA
715376
Salmonella enterica
23S rRNA pseudouridine
-
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine
pseudouridine synthase is responsible for the pseudouridine modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA
715376
Escherichia coli
23S rRNA pseudouridine
-
-
-
?
23S rRNA uridine
pseudouridine synthase is responsible for the pseudouridine modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA
715376
Salmonella enterica
23S rRNA pseudouridine
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function. Deletion of rluD is associated with extremely slow growth, increased readthrough of stop codons, and defects in 50S ribosomal subunit assembly and 30S-50S subunit association
Escherichia coli
malfunction
inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function
Salmonella enterica
General Information (protein specific)
General Information
Commentary
Organism
malfunction
inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function. Deletion of rluD is associated with extremely slow growth, increased readthrough of stop codons, and defects in 50S ribosomal subunit assembly and 30S-50S subunit association
Escherichia coli
malfunction
inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function
Salmonella enterica
Other publictions for EC 5.4.99.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730948
Kamalampeta
Archaeal proteins Nop10 and Ga ...
Pyrococcus furiosus
Sci. Rep.
2
663
2012
2
-
1
-
-
-
-
-
-
-
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-
-
1
-
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1
-
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2
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1
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1
-
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-
-
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-
-
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-
-
-
-
-
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1
1
-
-
-
714379
Kipper
Pseudouridylation of 23S rRNA ...
Escherichia coli
Biochimie
93
834-844
2011
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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715376
O’Connor
Inactivation of the RluD pseud ...
Escherichia coli, Salmonella enterica
J. Bacteriol.
193
154-162
2011
-
-
-
-
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2
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2
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2
2
-
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-
716902
Blaby
Pseudouridine formation in arc ...
Haloferax volcanii, Haloferax volcanii DSM 3757, Haloferax volcanii H26
RNA
17
1367-1380
2011
-
-
-
-
-
-
-
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-
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2
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13
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4
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2
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4
-
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2
4
-
-
-
715198
Sato
Genetic analyses of the antibi ...
Bifidobacterium bifidum, Bifidobacterium bifidum Yakult YIT 4001, no activity in Bifidobacterium bifidum strain Yakult YIT 4007
Int. J. Food Microbiol.
137
254-258
2010
-
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2
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3
-
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2
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2
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716899
Ero
Specificity and kinetics of 23 ...
Escherichia coli
RNA
16
2075-2084
2010
-
-
1
-
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1
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1
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1
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3
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1
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1
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1
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3
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1
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1
1