BRENDA - Enzyme Database show
show all sequences of 5.4.99.B4

Pseudouridylation of 23S rRNA helix 69 promotes peptide release by release factor RF2 but not by release factor RF1

Kipper, K.; Sild, S.; Hetenyi, C.; Remme, J.; Liiv, A.; Biochimie 93, 834-844 (2011)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-agarose column chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine
RluD is a helix 69-specific enzyme. The yield of pseudouridylation by RluD is 2.1 pseudouridines formed in helix 69 of 23S rRNA
714379
Escherichia coli
23S rRNA pseudouridine
-
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-agarose column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
23S rRNA uridine
RluD is a helix 69-specific enzyme. The yield of pseudouridylation by RluD is 2.1 pseudouridines formed in helix 69 of 23S rRNA
714379
Escherichia coli
23S rRNA pseudouridine
-
-
-
?
Other publictions for EC 5.4.99.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730948
Kamalampeta
Archaeal proteins Nop10 and Ga ...
Pyrococcus furiosus
Sci. Rep.
2
663
2012
2
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1
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1
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1
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2
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1
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1
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1
1
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-
714379
Kipper
Pseudouridylation of 23S rRNA ...
Escherichia coli
Biochimie
93
834-844
2011
-
-
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-
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1
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1
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1
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1
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1
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715376
O’Connor
Inactivation of the RluD pseud ...
Escherichia coli, Salmonella enterica
J. Bacteriol.
193
154-162
2011
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2
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2
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2
2
-
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716902
Blaby
Pseudouridine formation in arc ...
Haloferax volcanii, Haloferax volcanii DSM 3757, Haloferax volcanii H26
RNA
17
1367-1380
2011
-
-
-
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2
-
13
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4
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2
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4
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2
4
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715198
Sato
Genetic analyses of the antibi ...
Bifidobacterium bifidum, Bifidobacterium bifidum Yakult YIT 4001, no activity in Bifidobacterium bifidum strain Yakult YIT 4007
Int. J. Food Microbiol.
137
254-258
2010
-
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2
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3
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2
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2
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2
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716899
Ero
Specificity and kinetics of 23 ...
Escherichia coli
RNA
16
2075-2084
2010
-
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1
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1
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1
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1
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3
-
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1
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1
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1
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1
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3
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1
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1
1