Literature summary for 5.4.99.B22 extracted from

Simos, G.; Tekotte, H.; Grosjean, H.; Segref, A.; Sharma, K.; Tollervey, D.; Hurt, E.C.
Nuclear pore proteins are involved in the biogenesis of functional tRNA (1996), EMBO J., 15, 2270-2284.

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Cloned(Commentary)

Cloned (Comment)	Organism
the PUS1 ORF is tagged at its amino-terminal end with six histidines and overexpressed in Escherichia coli	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	Pus1p is an intranuclear protein	Saccharomyces cerevisiae	5634	-

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12211	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tRNAIle(UAU) uridine27	intron-independent reaction	Saccharomyces cerevisiae	tRNAIle(UAU) pseudouridine27	-	?
tRNAIle(UAU) uridine34	intron-dependent reaction	Saccharomyces cerevisiae	tRNAIle(UAU) pseudouridine34	-	?
tRNAIle(UAU) uridine36	intron-dependent reaction	Saccharomyces cerevisiae	tRNAIle(UAU) pseudouridine36	-	?

Synonyms

Synonyms	Comment	Organism
Pus1p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)