Protein Variants | Comment | Organism |
---|---|---|
R116A | the mutant exhibits some activity with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate | Homo sapiens |
R116C | the activity of the mutant enzyme with the yeast pre-tRNAIle substrate is approximately one-third that of the wild-type levels. No activity with the mouse tRNAMet substrate | Homo sapiens |
R116E | the mutant is inactive with with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate | Homo sapiens |
R116G | the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate | Homo sapiens |
R116H | the mutant is inactive with with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate | Homo sapiens |
R116K | the activity of the mutant enzyme with the yeast pre-tRNAIle substrate is approximately one-third that of the wild-type levels | Homo sapiens |
R116N | the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate | Homo sapiens |
R116Q | the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate | Homo sapiens |
R116S | the mutant exhibits some activity with the yeast pre-tRNAIle substrate. No activity with the mouse tRNAMet substrate | Homo sapiens |
R116W | the mutant is inactive with with the yeast pre-tRNAIle substrate. Low activity with the mouse tRNAMet substrate | Homo sapiens |
Y173C | mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low | Homo sapiens |
Y173F | mutant enzyme is minimally activity with yeast pre-tRNAIle | Homo sapiens |
Y173F | mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low | Homo sapiens |
Y173G | mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low | Homo sapiens |
Y173S | mutant enzyme is minimally activity with yeast pre-tRNAIle | Homo sapiens |
Y173T | mutant enzyme is minimally activity with yeast pre-tRNAIle | Homo sapiens |
Y173T | mutant enzyme retains some activity with yeast pre-tRNAIle. The activity is relatively low | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000032 | - |
yeast pre-tRNAIle uridine | possible modification sites are uridine 27, uridine30, uridine34 or uridine36, wild-type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
0.000168 | - |
yeast pre-tRNAIle uridine | possible modification sites are uridine 27, uridine30, uridine34 or uridine36, mutant enzyme R116K, pH and temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y606 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human tRNAMet uridine27 | - |
Homo sapiens | human tRNAMet pseudouridine27 | - |
? | |
additional information | Pus1p modifies uridines at positions 1, 26, 27, 28, 30, 34, 36, 65, and 67 depending on the source of the enzyme (Saccharomyces cerevisiae or mouse), the substrates (intron-containing or not), or whether the activity is monitored in vivo or in vitro. Out of all the positions that Pus1p modifies, the modification of uridines at positions 27 and 28 is by far the most common in tRNAs, with pseudouridine infrequently found at the other positions that the Pus1p enzymes recognize | Homo sapiens | ? | - |
? | |
mouse tRNAIle(UAU) uridine30 | - |
Homo sapiens | mouse tRNAIle(UAU) pseudouridine30 | - |
? | |
mouse tRNAMet uridine27 | - |
Homo sapiens | mouse tRNAMet pseudouridine27 | - |
? | |
yeast pre-tRNAIle uridine | possible modification sites are uridine 27, uridine30, uridine34 or uridine36 | Homo sapiens | yeast pre-tRNAIle pseudouridine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hPus1p | - |
Homo sapiens |
pseudouridine synthase 1 | - |
Homo sapiens |