Literature summary for 5.4.99.B22 extracted from

Arluison, V.; Hountondji, C.; Robert, B.; Grosjean, H.
Transfer RNA-Pseudouridine Synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition (1998), Biochemistry, 37, 7268-7276.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zinc	contains one atom of zinc per 63000 Da monomer. This zinc ion could not be removed by treatment with EDTA or urea. However, a zinc-depleted enzyme is obtained after prolonged dialysis against the specific chelating agent 1,10-phenanthroline. Removal of the zinc ion results in inactivation of the enzyme with concomitant loss of its ability to bind tRNA. Structural role for the zinc in the tRNA-pseudouridine synthetase Pus1	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12211	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
PUS1	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)