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Literature summary for 5.4.99.9 extracted from
- Identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay (2017), Biochem. Biophys. Res. Commun., 493, 58-63 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ugmA, recombinant expression of His8-tagged enzyme in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| flavopiridol | a compound that increases the melting temperature of Aspergillus fumigatus UGM. Flavopiridol also is a non-competitive inhibitor of UGM, and docking studies reveal that flavopiriol binds in the adenine-galactopyranose pocket and interacts with several residues. This compound does not inhibit the prokaryotic UGM from Mycobacteria tuberculosis. Inhibition mechanism of flavopiriodol | Aspergillus fumigatus |  |
| additional information | identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay, docking studies | Aspergillus fumigatus | |
| additional information | identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay. Flavopiridol does not inhibit the prokaryotic UGM from Mycobacterium tuberculosis, in contrast to the eukaryotic enzyme from Aspergillus fumigatus | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactopyranose | Mycobacterium tuberculosis | - |
UDP-alpha-D-galactofuranose | - |
r | |
| UDP-alpha-D-galactopyranose | Aspergillus fumigatus | - |
UDP-alpha-D-galactofuranose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q4W1X2 | i.e. Neosartorya fumigata | - |
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His8-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage with TEV protease, dialysis, and another step of nickel affinity chromatography and dialysis | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactopyranose | - |
Mycobacterium tuberculosis | UDP-alpha-D-galactofuranose | - |
r | |
| UDP-alpha-D-galactopyranose | - |
Aspergillus fumigatus | UDP-alpha-D-galactofuranose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AfUGM | - |
Aspergillus fumigatus |
| MtUGM | - |
Mycobacterium tuberculosis |
| UDP-Galp mutase | - |
Mycobacterium tuberculosis |
| UDP-Galp mutase | - |
Aspergillus fumigatus |
| UGM | - |
Mycobacterium tuberculosis |
| UGM | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aspergillus fumigatus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.454 | - |
flavopiridol | pH 7.0, 25°C, recombinant detagged enzyme | Aspergillus fumigatus | |
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Release 2023.1 (January 2023)
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