Cloned (Comment) | Organism |
---|---|
gene glf, overexpression of C-terminally His6-tagged enzyme in Escherichia coi strain BL21 StarTM (DE3) | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactopyranose | Mycobacterium tuberculosis | - |
UDP-alpha-D-galactofuranose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coi strain BL21 StarTM (DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose | reaction mechanism, overview. FAD-UDP-alpha-D-galactopyranose/UDP-alpha-D-galactofuranose adduct as an intermediate in the catalytic cycle, the chemical mechanism for UGM involves an SN2-type displacement of UDP from UDP-alpha-D-galactopyranose/UDP-alpha-D-galactofuranose by N5 of FADred | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactopyranose | - |
Mycobacterium tuberculosis | UDP-alpha-D-galactofuranose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
UGM | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | reduced FAD is required for activity. Reconstitution of apoenzyme with other FAD analogues, e.g. with methoxy-FAD, trifluoromethyl-FAD, or chloro-FAD, which are less effective than FAD, overview | Mycobacterium tuberculosis |