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Literature summary for 5.4.99.9 extracted from

  • Sun, H.G.; Ruszczycky, M.W.; Chang, W.C.; Thibodeaux, C.J.; Liu, H.W.
    Nucleophilic participation of reduced flavin coenzyme in mechanism of UDP-galactopyranose mutase (2012), J. Biol. Chem., 287, 4602-4608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene glf, overexpression of C-terminally His6-tagged enzyme in Escherichia coi strain BL21 StarTM (DE3) Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactopyranose Mycobacterium tuberculosis
-
UDP-alpha-D-galactofuranose
-
r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coi strain BL21 StarTM (DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose reaction mechanism, overview. FAD-UDP-alpha-D-galactopyranose/UDP-alpha-D-galactofuranose adduct as an intermediate in the catalytic cycle, the chemical mechanism for UGM involves an SN2-type displacement of UDP from UDP-alpha-D-galactopyranose/UDP-alpha-D-galactofuranose by N5 of FADred Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactopyranose
-
Mycobacterium tuberculosis UDP-alpha-D-galactofuranose
-
r

Synonyms

Synonyms Comment Organism
UGM
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FADH2 reduced FAD is required for activity. Reconstitution of apoenzyme with other FAD analogues, e.g. with methoxy-FAD, trifluoromethyl-FAD, or chloro-FAD, which are less effective than FAD, overview Mycobacterium tuberculosis