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Literature summary for 5.4.99.9 extracted from

  • Penman, G.A.; Lockhart, D.E.; Ferenbach, A.; van Aalten, D.M.
    Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus (2012), Acta Crystallogr. Sect. F, 68, 705-708.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is a potential drug target involved in the synthesis of the cell wall of this fungal pathogen Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, and in strain B893 (DE3) pLysS for the selnomethionine-labeled enzyme Aspergillus fumigatus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 500 nl 15 mg/ml protein solution with 500 nl reservoir solution, containing 0.1 M HEPES, pH 7.5, 20% PEG 3350, 0.4 M ammonium sulfate, 4% formamide, and equilibration against 0.07 ml reservoir solution, for enzyme complex crystals, the enzyme is incubated for 10 min on ice with various substrate/inhibitor/reducing ligands 10 mM UDP-Galp, 10 mM UDP, 5 mM UDP-glucose, 5-20 mM sodium dithionite, respectively, before crystallization, 2 days, X-ray diffraction structure determination and analysis at 3.25 A resolution. Incorporation of selenomethionine is achieved, but the resulting crystals do not allow solution of the phase problem Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57200
-
x * 57200, about, sequence calculation Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4W1X2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS or B893 (DE3) pLysS by glutathione affinity chroatography and gel filtration Aspergillus fumigatus

Subunits

Subunits Comment Organism
? x * 57200, about, sequence calculation Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
UGM
-
Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the synthesis of the cell wall Aspergillus fumigatus