Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a potential drug target involved in the synthesis of the cell wall of this fungal pathogen | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, and in strain B893 (DE3) pLysS for the selnomethionine-labeled enzyme | Aspergillus fumigatus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 500 nl 15 mg/ml protein solution with 500 nl reservoir solution, containing 0.1 M HEPES, pH 7.5, 20% PEG 3350, 0.4 M ammonium sulfate, 4% formamide, and equilibration against 0.07 ml reservoir solution, for enzyme complex crystals, the enzyme is incubated for 10 min on ice with various substrate/inhibitor/reducing ligands 10 mM UDP-Galp, 10 mM UDP, 5 mM UDP-glucose, 5-20 mM sodium dithionite, respectively, before crystallization, 2 days, X-ray diffraction structure determination and analysis at 3.25 A resolution. Incorporation of selenomethionine is achieved, but the resulting crystals do not allow solution of the phase problem | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57200 | - |
x * 57200, about, sequence calculation | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4W1X2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS or B893 (DE3) pLysS by glutathione affinity chroatography and gel filtration | Aspergillus fumigatus |
Subunits | Comment | Organism |
---|---|---|
? | x * 57200, about, sequence calculation | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
UGM | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoenzyme | Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the synthesis of the cell wall | Aspergillus fumigatus |