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Literature summary for 5.4.99.9 extracted from

  • Partha, S.K.; Sadeghi-Khomami, A.; Slowski, K.; Kotake, T.; Thomas, N.R.; Jakeman, D.L.; Sanders, D.A.
    Chemoenzymatic synthesis, inhibition studies, and X-ray crystallographic analysis of the phosphono analog of UDP-Galp as an inhibitor and mechanistic probe for UDP-galactopyranose mutase (2010), J. Mol. Biol., 403, 578-590.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43 cells Klebsiella pneumoniae
expressed in Escherichia coli C43 cells Mycobacterium tuberculosis
expressed in Escherichia coli C43 cells Deinococcus radiodurans

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with UDP-CH2-Galp, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 6.5), 0.2 M LiCl, and 28% (w/v) polyethylene glycol 6000 Deinococcus radiodurans

Inhibitors

Inhibitors Comment Organism Structure
UDP-CH2-Galp moderate inhibition of UGM Deinococcus radiodurans
UDP-CH2-Galp moderate inhibition of UGM Klebsiella pneumoniae
UDP-CH2-Galp moderate inhibition of UGM Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans Q9RYF1
-
-
Klebsiella pneumoniae
-
-
-
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Protino Ni-IDA 1000 column chromatography Klebsiella pneumoniae
Protino Ni-IDA 1000 column chromatography Mycobacterium tuberculosis
Protino Ni-IDA 1000 column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactopyranose
-
Klebsiella pneumoniae UDP-alpha-D-galactofuranose
-
r
UDP-alpha-D-galactopyranose
-
Mycobacterium tuberculosis UDP-alpha-D-galactofuranose
-
r
UDP-alpha-D-galactopyranose
-
Deinococcus radiodurans UDP-alpha-D-galactofuranose
-
r

Synonyms

Synonyms Comment Organism
galactopyranose mutase
-
Klebsiella pneumoniae
galactopyranose mutase
-
Mycobacterium tuberculosis
galactopyranose mutase
-
Deinococcus radiodurans
UGM
-
Klebsiella pneumoniae
UGM
-
Mycobacterium tuberculosis
UGM
-
Deinococcus radiodurans

Cofactor

Cofactor Comment Organism Structure
FAD
-
Klebsiella pneumoniae
FAD
-
Mycobacterium tuberculosis
FAD
-
Deinococcus radiodurans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.411
-
in 50 mM sodium phosphate buffer (pH 7.0), at 22°C Deinococcus radiodurans UDP-CH2-Galp
0.479
-
in 50 mM sodium phosphate buffer (pH 7.0), at 22°C Klebsiella pneumoniae UDP-CH2-Galp
0.495
-
in 50 mM sodium phosphate buffer (pH 7.0), at 22°C Mycobacterium tuberculosis UDP-CH2-Galp