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Literature summary for 5.4.99.9 extracted from

  • Oppenheimer, M.; Valenciano, A.L.; Sobrado, P.
    Isolation and characterization of functional Leishmania major virulence factor UDP-galactopyranose mutase (2011), Biochem. Biophys. Res. Commun., 407, 552-556.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21T1 and BL21(DE3) cells Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.087
-
 UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54960
-
 1 * 54960, calculated from amino acid sequence Leishmania major
55000
-
 1 * 55000, SDS-PAGE Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel immobilized metal affinity chromatography, DEAE column chromatography, and purification with HaloLink resin Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is active only in the reduced state Leishmania major ?
-
 ?
UDP-D-galactofuranose
-
 Leishmania major UDP-D-galactopyranose
-
 r
UDP-D-galactopyranose
-
 Leishmania major UDP-D-galactofuranose
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Leishmania major
monomer 1 * 54960, calculated from amino acid sequence Leishmania major

Synonyms

Synonyms Comment Organism
UGM
-
 Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Leishmania major

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Leishmania major

General Information

General Information Comment Organism
physiological function UDP-galactopyranose mutase is a virulence factor in Leishmania major Leishmania major

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
57
-
 UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Leishmania major