Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.9 extracted from

  • Oppenheimer, M.; Poulin, M.B.; Lowary, T.L.; Helm, R.F.; Sobrado, P.
    Characterization of recombinant UDP-galactopyranose mutase from Aspergillus fumigatus (2010), Arch. Biochem. Biophys., 502, 31-38.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21T1 cells Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Aspergillus fumigatus
57152
-
4 * 57152, calculated from amino acid sequence Aspergillus fumigatus
228600
-
calculated from amino acid sequence Aspergillus fumigatus
275000
-
gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel immobilized affinity chromatography and DEAE column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is active in the oxidized state, being 2-3fold less active than in the reduced state. In the oxidized state, UGM does not bind UDP-galactopyranose Aspergillus fumigatus ?
-
?
UDP-D-galactofuranose
-
Aspergillus fumigatus UDP-D-galactopyranose
-
r
UDP-D-galactopyranose
-
Aspergillus fumigatus UDP-D-galactofuranose
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 55000, SDS-PAGE Aspergillus fumigatus
homotetramer 4 * 57152, calculated from amino acid sequence Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
UGM
-
Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
72
-
UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
650
-
UDP-D-galactofuranose in 25 mM HEPES, 125 mM NaCl, 20 mM dithionite, pH 7.5, at 37°C Aspergillus fumigatus