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Literature summary for 5.4.99.8 extracted from

  • Calegario, G.; Pollier, J.; Arendt, P.; de Oliveira, L.S.; Thompson, C.; Soares, A.R.; Pereira, R.C.; Goossens, A.; Thompson, F.L.
    Cloning and functional characterization of cycloartenol synthase from the red seaweed Laurencia dendroidea (2016), PLoS ONE, 11, e0165954 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.8

Cloned(Commentary)

Cloned (Comment) Organism
gene LdCAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in a sterol-engineered Saccharomyces cerevisiae strains PA14, TM097, and TM122, that are derived from strain TM1 in the S288c / BY4742 background. Yeast strain PA14 is derived from strain TM1 by knocking out the TRP1 gene using CRISPR/Cas9 Laurencia dendroidea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Laurencia dendroidea
-
 cycloartenol
-
 r

Organism

Organism UniProt Comment Textmining
Laurencia dendroidea A0A1I9Q605
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Laurencia dendroidea cycloartenol
-
 r

Synonyms

Synonyms Comment Organism
CAS
-
 Laurencia dendroidea
LdCAS
-
 Laurencia dendroidea

General Information

General Information Comment Organism
evolution the enzyme belongs to the family of 2,3-oxidosqualene cyclases (OSCs). A key step in the synthesis of cholesterol in red algae may be the reduction of the C-24(25) double bond by a DHCR24-like enzyme Laurencia dendroidea
metabolism profiling of Laurencia dendroidea reveals cholesterol as the major sterol accumulating in this species, implicating red seaweeds contain a hybrid sterol synthesis pathway in which the phytosterol precursor cycloartenol is converted into the major animal sterol cholesterol Laurencia dendroidea
additional information steroid profiling by GC-MS analysis Laurencia dendroidea