Literature summary for 5.4.99.7 extracted from

Kumar, A.; Fogelman, E.; Weissberg, M.; Tanami, Z.; Veilleux, R.E.; Ginzberg, I.
Lanosterol synthase-like is involved with differential accumulation of steroidal glycoalkaloids in potato (2017), Planta, 246, 1189-1202 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene LAS1, recombinant expression in Solanum tuberosum leaves using the transformation method with Agrobacterium tumefaciens strain EHA 105. Overexpression of the Arabidopsis LAS in transgenic potato modifies phytosterol and steroidal glycoalkaloid metabolite levels and the transcript levels of the respective biosynthetic genes. LAS1 pverexpression in Solanum tnerosum leaves results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced	Arabidopsis thaliana
identification of a putative LAS gene from potato (StLAS-like) and OSC activity verification in a yeast complementation assay by expression of the potato enzyme in LAS-deficient Saccharomyces cerevisiae strain GIL77. Analyses of StLAS-like transcript levels and StLAS-like::GUS reporter assays indicate different spatial expression in potato. Promoter sequence and activity analysis	Solanum tuberosum

Cloned (Comment)

Organism

gene LAS1, recombinant expression in Solanum tuberosum leaves using the transformation method with Agrobacterium tumefaciens strain EHA 105. Overexpression of the Arabidopsis LAS in transgenic potato modifies phytosterol and steroidal glycoalkaloid metabolite levels and the transcript levels of the respective biosynthetic genes. LAS1 pverexpression in Solanum tnerosum leaves results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced

Arabidopsis thaliana

identification of a putative LAS gene from potato (StLAS-like) and OSC activity verification in a yeast complementation assay by expression of the potato enzyme in LAS-deficient Saccharomyces cerevisiae strain GIL77. Analyses of StLAS-like transcript levels and StLAS-like::GUS reporter assays indicate different spatial expression in potato. Promoter sequence and activity analysis

Solanum tuberosum

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of Arabidopsis thaliana AtLAS in leaves via transformation by Agrobacterium tumefaciens strain EHA 105, where the endogenic StLAS-like is not expressed, results in increased steroidal glycoalkaloid level and reduced phytosterol level in the leaves, while the steroidal glycoalkaloid level in the tuber flesh is reduced	Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene	Arabidopsis thaliana	-	lanosterol	-	r
(3S)-2,3-epoxy-2,3-dihydrosqualene	Solanum tuberosum	-	lanosterol	-	r

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q1G1A4	-	-
Solanum tuberosum	A0A1B0YWV6	cv. Desiree	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
tuber	-	Solanum tuberosum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene	-	Arabidopsis thaliana	lanosterol	-	r
(3S)-2,3-epoxy-2,3-dihydrosqualene	-	Solanum tuberosum	lanosterol	-	r

Synonyms

Synonyms	Comment	Organism
AtLAS	-	Arabidopsis thaliana
LAS	-	Arabidopsis thaliana
LAS	-	Solanum tuberosum
LAS1	-	Arabidopsis thaliana
tuber-specific StLAS-like	-	Solanum tuberosum

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the product-specific oxidosqualene cyclases (OSCs) that constitute a significant metabolic branch point which is the initial step of triterpene chemical diversity	Arabidopsis thaliana
evolution	the enzyme belongs to the product-specific oxidosqualene cyclases (OSCs) that constitute a significant metabolic branch point which is the initial step of triterpene chemical diversity	Solanum tuberosum
metabolism	phytosterol homeostasis may be maintained in leaves through diversion of intermediates into glycoalkaloid biosynthesis, whereas in tuber flesh, excess intermediates are catalyzed by tuber-specific StLAS-like, resulting in low tuber glycoalkaloids. The enzyme as product-specific oxidosqualene cyclase constitutes a significant metabolic branch point which is the initial step of triterpene chemical diversity	Solanum tuberosum
metabolism	the enzyme as product-specific oxidosqualene cyclase constitutes a significant metabolic branch point which is the initial step of triterpene chemical diversity	Arabidopsis thaliana