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Literature summary for 5.4.99.7 extracted from
- Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase (2012), Org. Lett., 14, 5222-5225.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q450H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme produng exclusively achilleol A as product | Saccharomyces cerevisiae |
| Q450H/V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing lanosterol, (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol, and protosta-16,24-dien-3beta-ol | Saccharomyces cerevisiae |
| T384Y | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing lanosterol, parkeol, and 9beta-lanosta-7,24-dien-3beta-ol | Saccharomyces cerevisiae |
| T384Y/Q450H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly parkeol and very low amounts of 9beta-lanosta-7,24-dien-3beta-ol and lanosterol | Saccharomyces cerevisiae |
| T384Y/Q450H/V454I | site-directed mutagenesis, the mutant produces exclusively reaction intermediate parkeol but not lanosterol as the sole end product | Saccharomyces cerevisiae |
| T384Y/V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly parkeol and lower amounts of 9beta-lanosta-7,24-dien-3beta-ol and lanosterol | Saccharomyces cerevisiae |
| V454I | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme producing mainly lanosterol and a low amount of (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of wild-type enzyme | Saccharomyces cerevisiae | lanosterol | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant Q450H | Saccharomyces cerevisiae | achilleol A | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant V454I | Saccharomyces cerevisiae | lanosterol + (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant Q450H/V454I | Saccharomyces cerevisiae | lanosterol + (13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol + protosta-16,24-dien-3beta-ol | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutants T384Y, T384Y/Q450H, and T384Y/V454I | Saccharomyces cerevisiae | lanosterol + parkeol + 9beta-lanosta-7,24-dien-3beta-ol | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant T384Y/Q450H/V454I | Saccharomyces cerevisiae | parkeol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ERG7 | - |
Saccharomyces cerevisiae |
| OSC | - |
Saccharomyces cerevisiae |
| Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |
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