Literature summary for 5.4.99.7 extracted from

Oliaro-Bosso, S.; Schulz-Gasch, T.; Balliano, G.; Viola, F.
Access of the substrate to the active site of yeast oxidosqualene cyclase: an inhibition and site-directed mutagenesis approach (2005), ChemBioChem, 6, 2221-2228.

Search for more literature for 5.4.99.7

Cloned(Commentary)

Cloned (Comment)	Organism
-	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
C457D	less active than the wild-type	Saccharomyces cerevisiae
C457D/A525C	similar activity as the wild-type	Saccharomyces cerevisiae
C457D/E526A	decrease in enzyme functionality	Saccharomyces cerevisiae
C457D/E526C	very sensitive to the thiol-reacting agent dodecylmaleimide, specific activity and thermal stability are severely reduced	Saccharomyces cerevisiae
C457D/E526D	no effect on catalytic avtivity	Saccharomyces cerevisiae
C457D/E526Q	no effect on catalytic avtivity	Saccharomyces cerevisiae

General Stability

General Stability	Organism
mutants C457D/E526C and C457D/E526A are not stable at 35°C	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
dodecyl-maleimide	mutant C457D shows 41.6% inhibition at 1 mM, 10.0% at 0.2 mM, and 7.2% at 0.025 mM, mutant C457D/E526A shows 9.0% inhibition at 0.2 mM and 0.0% at 0.025 mM, mutant C457D/A525C shows 86.0% inhibition at 1 mM, 30.5% at 0.2 mM, and 1.0% at 0.025 mM, mutant C457D/E526C shows 89.6% inhibition at 0.2 mM, 74.7% at 0.025 mM, and 45.1% at 0.01 mM	Saccharomyces cerevisiae
N,N-Dimethylformamide	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3S)-2,3-oxidosqualene	Saccharomyces cerevisiae	-	lanosterol	-	?
(3S)-2,3-oxidosqualene	Saccharomyces cerevisiae SMY8	-	lanosterol	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae SMY8	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0000015	-	mutant C457/E526A at 25°C	Saccharomyces cerevisiae
0.0000015	-	mutant C457/E526C at 25°C	Saccharomyces cerevisiae
0.0000025	-	mutant C457/E526C at 35°C	Saccharomyces cerevisiae
0.0000048	-	mutant C457/E526A at 35°C	Saccharomyces cerevisiae
0.0000063	-	mutant C457 at 25°C	Saccharomyces cerevisiae
0.0000087	-	mutant C457/E526D at 25°C	Saccharomyces cerevisiae
0.0000097	-	mutant C457/E526Q at 25°C	Saccharomyces cerevisiae
0.0000113	-	mutant C457/E525C at 25°C	Saccharomyces cerevisiae
0.0000128	-	mutant C457/E526D at 35°C	Saccharomyces cerevisiae
0.0000172	-	wild-type, at 25°C	Saccharomyces cerevisiae
0.0000192	-	mutant C457/E526Q at 35°C	Saccharomyces cerevisiae
0.000021	-	mutant C457/E525C at 35°C	Saccharomyces cerevisiae
0.0000213	-	mutant C457 at 35°C	Saccharomyces cerevisiae
0.0000292	-	wild-type, at 35°C	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S)-2,3-oxidosqualene	-	Saccharomyces cerevisiae	lanosterol	-	?
(3S)-2,3-oxidosqualene	-	Saccharomyces cerevisiae SMY8	lanosterol	-	?
squalene	-	Saccharomyces cerevisiae	?	-	?
squalene	-	Saccharomyces cerevisiae SMY8	?	-	?

Synonyms

Synonyms	Comment	Organism
lanosterol synthase	-	Saccharomyces cerevisiae
OSC	-	Saccharomyces cerevisiae
Oxidosqualene cyclase	-	Saccharomyces cerevisiae
SceOSC	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)