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Literature summary for 5.4.99.5 extracted from

  • Perez, E.; Rubio, M.B.; Cardoza, R.E.; Gutierrez, S.; Bettiol, W.; Monte, E.; Hermosa, R.
    The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei (2015), Front. Microbiol., 6, 1181 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Tparo7, DNA and amino acid sequence determination and analysis, quantitative real-time PCR enzyme expression analysis Trichoderma parareesei

Protein Variants

Protein Variants Comment Organism
additional information silencing of gene Tparo7, mutant phenotype, plant rhizosphere colonization tests on tomato plants, detailed overview Trichoderma parareesei

Organism

Organism UniProt Comment Textmining
Trichoderma parareesei A0A0S0FTT9 formerly Trichoderma reesei
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Trichoderma parareesei IMI 113135 / T6 A0A0S0FTT9 formerly Trichoderma reesei
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Source Tissue

Source Tissue Comment Organism Textmining
mycelium
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Trichoderma parareesei
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Synonyms

Synonyms Comment Organism
aro7
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Trichoderma parareesei
Tparo7
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Trichoderma parareesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Trichoderma parareesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Trichoderma parareesei

General Information

General Information Comment Organism
malfunction compared to wild-type enzyme, decreased levels of Tparo7 expression in the silenced transformants are accompanied by reduced chorismate mutase activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani strain 19, Fusarium oxysporum strain CECT 2866, and Botrytis cinerea strain B05.10 in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid are detected in supernatants from the silenced transformants, which are able to inhibit the growth of Fusarium oxysporum and Botrytis cinerea. In in vitro plant assays, Tparo7-silenced transformants also show a reduced capacity to colonize tomato roots. The growth of tomato plants colonized by the silenced transformants is reduced and the plants exhibit an increased susceptibility to Bortrytis cinerea in comparison with the responses observed for control plants. In addition, the plants turn yellowish and are defective in jasmonic acid- and ethylene-regulated signaling pathways which is seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes Trichoderma parareesei
metabolism the enzyme plays a central branch point role in the shikimate pathway, pathway and regulation, overview Trichoderma parareesei
physiological function enzyme chorismate mutase is a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites Trichoderma parareesei