Cloned (Comment) | Organism |
---|---|
gene Tparo7, DNA and amino acid sequence determination and analysis, quantitative real-time PCR enzyme expression analysis | Trichoderma parareesei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | silencing of gene Tparo7, mutant phenotype, plant rhizosphere colonization tests on tomato plants, detailed overview | Trichoderma parareesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma parareesei | A0A0S0FTT9 | formerly Trichoderma reesei | - |
Trichoderma parareesei IMI 113135 / T6 | A0A0S0FTT9 | formerly Trichoderma reesei | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trichoderma parareesei | - |
Synonyms | Comment | Organism |
---|---|---|
aro7 | - |
Trichoderma parareesei |
Tparo7 | - |
Trichoderma parareesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trichoderma parareesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trichoderma parareesei |
General Information | Comment | Organism |
---|---|---|
malfunction | compared to wild-type enzyme, decreased levels of Tparo7 expression in the silenced transformants are accompanied by reduced chorismate mutase activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani strain 19, Fusarium oxysporum strain CECT 2866, and Botrytis cinerea strain B05.10 in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid are detected in supernatants from the silenced transformants, which are able to inhibit the growth of Fusarium oxysporum and Botrytis cinerea. In in vitro plant assays, Tparo7-silenced transformants also show a reduced capacity to colonize tomato roots. The growth of tomato plants colonized by the silenced transformants is reduced and the plants exhibit an increased susceptibility to Bortrytis cinerea in comparison with the responses observed for control plants. In addition, the plants turn yellowish and are defective in jasmonic acid- and ethylene-regulated signaling pathways which is seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes | Trichoderma parareesei |
metabolism | the enzyme plays a central branch point role in the shikimate pathway, pathway and regulation, overview | Trichoderma parareesei |
physiological function | enzyme chorismate mutase is a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites | Trichoderma parareesei |