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Literature summary for 5.4.99.5 extracted from
- Crystal structure of chorismate mutase from Burkholderia thailandensis (2018), Acta Crystallogr. Sect. F, 74, 294-299 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene BTH_I1596, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)R3 Rosetta | Burkholderia thailandensis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme, sitting drop vapor diffusion method, mixing of 400 nl of 20 mg/ml protein in 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol, and 1 mM TCEP, with 400 nl reservoir solution containing 20% w/v PEG 3350, 200 mM ammonium nitrate, and equilibration against 0.08 ml of reservoir solution, 17°C, X-ray diffraction structure determination and analysis at 2.15 A resolution | Burkholderia thailandensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Burkholderia thailandensis | - |
Prephenate | - |
? |  |
| Chorismate | Burkholderia thailandensis ATCC 700388 / DSM 13276 / CIP 106301 / E264 | - |
Prephenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia thailandensis | Q2SY64 | - |
- |
| Burkholderia thailandensis ATCC 700388 / DSM 13276 / CIP 106301 / E264 | Q2SY64 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)R3 Rosetta by nickel affinity chromatography, gel filtration, and ultrafiltration | Burkholderia thailandensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Burkholderia thailandensis | Prephenate | - |
? | |
| Chorismate | - |
Burkholderia thailandensis ATCC 700388 / DSM 13276 / CIP 106301 / E264 | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the high-resolution structure of chorismate mutase is determined in the monoclinic space group P21 with three homodimers per asymmetric unit. The overall structure of each protomer has the prototypical AroQgamma topology and shares conserved binding-cavity residues with other chorismate mutases. The AroQgamma topology is composed entirely of helices connected by short loops | Burkholderia thailandensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AroQ | - |
Burkholderia thailandensis |
| BTH_I1596 | - |
Burkholderia thailandensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme structure analysis, enzyme topology, and conserved residues in the substrate-binding sites of chorismate mutase, overview | Burkholderia thailandensis |
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Release 2023.1 (January 2023)
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