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Literature summary for 5.4.99.5 extracted from

  • Lassila, J.K.; Keeffe, J.R.; Oelschlaeger, P.; Mayo, S.L.
    Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity (2005), Protein Eng. Des. Sel., 18, 161-163.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
A32S increased catalytic efficiency Escherichia coli
D48I no activity Escherichia coli
I81L/V85I reduced catalytic efficiency, alters packing against the hydrophobic ring face of the reacting molecule Escherichia coli
L7I no significant effect on catalytic efficiency Escherichia coli
V35I increased KM and turnover values compared to wild type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
chorismate mutant A32S Escherichia coli
0.249
-
chorismate mutant L7I Escherichia coli
0.304
-
chorismate wild type Escherichia coli
0.365
-
chorismate mutant V35I Escherichia coli
0.669
-
chorismate mutant I81L/V85I Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Escherichia coli
-
Prephenate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9J8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
Escherichia coli Prephenate
-
?

Synonyms

Synonyms Comment Organism
chorismate mutase
-
Escherichia coli
EcCM
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.4
-
chorismate mutant I81L/V85I Escherichia coli
36.55
-
chorismate mutant L7I Escherichia coli
38.87
-
chorismate wild type Escherichia coli
45.13
-
chorismate mutant A32S Escherichia coli
50.77
-
chorismate mutant V35I Escherichia coli