Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid | competitive inhibition | Mycobacterium tuberculosis | |
adamantane-1-phosphonate | no inhibitory effect up to concentrations of 0.1 and 1 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
chorismate | leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
0.18 | - |
chorismate | untagged enzyme, at 30°C and pH 7.5 | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | leaderless protein | Mycobacterium tuberculosis | 5737 | - |
periplasmic space | full-length protein | Mycobacterium tuberculosis | 42597 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18600 | - |
monomer, calculated, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
18600 | - |
monomer, mass spectrometry, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
19540 | - |
monomer, calculated from the variant that lacks 33 N-terminal residues | Mycobacterium tuberculosis |
19540 | - |
monomer, mass spectrometry | Mycobacterium tuberculosis |
19670 | - |
monomer, calculated from the leaderless variant with additional start methionine | Mycobacterium tuberculosis |
19670 | - |
monomer, mass, spectrometry, leaderless variant with additional start methionine | Mycobacterium tuberculosis |
23000 | - |
monomer, SDS-PAGE, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
35400 | - |
ultracentrifugation | Mycobacterium tuberculosis |
43400 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | Mycobacterium tuberculosis | - |
Prephenate | - |
? | |
Chorismate | Mycobacterium tuberculosis H37Rv | - |
Prephenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Renatured (Comment) | Organism |
---|---|
thermal denaturation shows a sharp transition from the native to the denatured state, with a calculated Tm of 48°C. Upon subsequent cooling, the protein renatured easily | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Mycobacterium tuberculosis | Prephenate | - |
? | |
Chorismate | - |
Mycobacterium tuberculosis H37Rv | Prephenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, ultracentrifugation, gel filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase | - |
Mycobacterium tuberculosis |
CM | - |
Mycobacterium tuberculosis |
MtCM | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
calculated Tm: 48°C | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
chorismate | untagged enzyme, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
56 | - |
chorismate | leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid | - |
Mycobacterium tuberculosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium tuberculosis | calculated | - |
4.9 |