Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
crystal structures of double mutants C88S/R90K and C88K/R90S, hanging drop vapour diffusion method at room temperature, space group R3 with a and b: 82.6 A and c: 42.8 A | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C88K/R90S | lower activity than wild-type enzyme | Bacillus subtilis |
C88S/R90K | lower activity than wild-type enzyme | Bacillus subtilis |
R90A | no activity detectable | Bacillus subtilis |
R90G | very low activity | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxabicyclic dicarboxylic acid | transition state analogon, competitive inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
chorismate | 30°C, pH 7, wild-type | Bacillus subtilis | |
0.15 | - |
chorismate | 30°C, pH 7, mutant R90G | Bacillus subtilis | |
1.9 | - |
chorismate | 30°C, pH 7, mutant C88K/R90S | Bacillus subtilis | |
4.3 | - |
chorismate | 30°C, pH 7, mutant C88S/R90K | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | Bacillus subtilis | first committed step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and higher plants | Prephenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P19080 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Bacillus subtilis | Prephenate | - |
? | |
Chorismate | first committed step in the biosynthesis of the aromatic amino acids tyrosine and phenylalanine in bacteria, fungi and higher plants | Bacillus subtilis | Prephenate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BsCM | - |
Bacillus subtilis |
chorismate mutase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
chorismate | 30°C, pH 7, mutant R90G | Bacillus subtilis | |
0.29 | - |
chorismate | 30°C, pH 7, mutant C88S/R90K | Bacillus subtilis | |
0.32 | - |
chorismate | 30°C, pH 7, mutant C88K/R90S | Bacillus subtilis | |
46 | - |
chorismate | 30°C, pH 7, wild-type | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
oxabicyclic dicarboxylic acid | >> 1 mM mutant C88S/R90K | Bacillus subtilis | |
0.003 | - |
oxabicyclic dicarboxylic acid | 30°C, pH 7, wild-type | Bacillus subtilis | |
0.004 | - |
oxabicyclic dicarboxylic acid | 30°C, pH 7, mutant R90G | Bacillus subtilis | |
1.1 | - |
oxabicyclic dicarboxylic acid | 30°C, pH 7, mutant C88K/R90S | Bacillus subtilis |