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Literature summary for 5.4.99.5 extracted from

  • Hertel, S.C.; Hieke, M.; Gröger, D.
    Purification and characterization of chorismate mutase isoenzymes from Ruta graveolens (1991), Acta Biotechnol., 11, 39-48.
No PubMed abstract available
Search for more literature for 5.4.99.5

Activating Compound

Activating Compound Comment Organism Structure
L-Trp enzyme form CM1 is activated Ruta graveolens
L-Trp enzyme form CM2 is unaffected Ruta graveolens

Inhibitors

Inhibitors Comment Organism Structure
4-Methyl-DL-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
6-Methyl-DL-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
Ba2+
-
 Ruta graveolens
Co2+
-
 Ruta graveolens
Cu2+ strong Ruta graveolens
DL-3-fluoro-Phe enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
DL-5-Fluoro-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
DL-5-hydroxy-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
Fe2+
-
 Ruta graveolens
Hg2+ HgCl2 Ruta graveolens
L-Phe
-
 Escherichia coli
L-Phe enzyme form CM1 is inhibited; Trp reverses inhibition Ruta graveolens
L-Tyr
-
 Escherichia coli
L-Tyr enzyme form CM1 is inhibited; enzyme form CM2 is not inhibited; Trp reverses inhibition Ruta graveolens
Mn2+
-
 Ruta graveolens
Pb2+
-
 Ruta graveolens
PCMB
-
 Ruta graveolens
Zn2+ strong Ruta graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 chorismate enzyme form CM2 Ruta graveolens
1.1
-
 chorismate enzyme CM1 Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 enzyme form CM2, gel filtration Ruta graveolens
56000
-
 enzyme form CM1, gel filtration Ruta graveolens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Ruta graveolens
-
 2 isoenzymes: CM1 and CM2
-

Purification (Commentary)

Purification (Comment) Organism
partial Ruta graveolens

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Ruta graveolens
-

Storage Stability

Storage Stability Organism
-20°C, enzyme form CM1 and CM2 are stable Ruta graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Escherichia coli Prephenate
-
 ?
Chorismate
-
 Ruta graveolens Prephenate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 37 enzyme form CM1 Ruta graveolens
56
-
 enzyme form CM2 Ruta graveolens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 5 min, enzyme form CM1, 50% loss of activity Ruta graveolens
50 60 5 min, enzyme form CM2, stable Ruta graveolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10 enzyme form CM2 Ruta graveolens
7.8
-
 enzyme form CM1 Ruta graveolens