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Literature summary for 5.4.99.5 extracted from
- Chorismate mutase from Streptomyces aureofaciens (1987), Methods Enzymol., 142, 463-472.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-Trp | no effect | Kitasatospora aureofaciens |  |
General Stability
| General Stability | Organism |
|---|---|
| chorismate mutase irreversibly immobilized by n-decylamine-substituted agarose allows repeated quantitative conversion of chorismate to prephenate | Kitasatospora aureofaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Tyr | no inhibition | Kitasatospora aureofaciens | |
| L-Tyr | no inhibition | Streptomyces venezuelae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
chorismate | - |
Kitasatospora aureofaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14500 | - |
x * 14500, at least 3 subunits not linked together by disulfide bridges, SDS-PAGE | Kitasatospora aureofaciens |
| 51000 | - |
sucrose density gradient centrifugation | Kitasatospora aureofaciens |
| 63000 | - |
gel filtration | Kitasatospora aureofaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kitasatospora aureofaciens | - |
- |
- |
| Streptomyces venezuelae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Kitasatospora aureofaciens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| renaturation of heat-denatured enzyme | Kitasatospora aureofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 300 | - |
- |
Kitasatospora aureofaciens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, in presence of PMSF, stable for at least 2 months | Kitasatospora aureofaciens |
| 4°C, in presence of PMSF, stable for at least 1 month | Kitasatospora aureofaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Streptomyces venezuelae | Prephenate | - |
? | |
| Chorismate | - |
Kitasatospora aureofaciens | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 14500, at least 3 subunits not linked together by disulfide bridges, SDS-PAGE | Kitasatospora aureofaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
reversible heat inactivation above | Kitasatospora aureofaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Kitasatospora aureofaciens |
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Release 2023.1 (January 2023)
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