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Literature summary for 5.4.99.4 extracted from

  • Kung, H.F.; Stadtman, T.C.
    Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase (1971), J. Biol. Chem., 246, 3378-3388.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl groups the enzyme is a sulfhydryl protein Eubacterium barkeri

General Stability

General Stability Organism
enzyme rapidly decays, when stored at low protein concentrations, 1 mg/ml. Complete loss of activity after 3 days at 0°C and 38% loss of activity after 6 days at -80°C Eubacterium barkeri

Inhibitors

Inhibitors Comment Organism Structure
1-Methyl-1,2-cis-cyclopropanedicarboxylate
-
Eubacterium barkeri
1-Methyl-1,2-trans-cyclopropanedicarboxylate
-
Eubacterium barkeri
glutaconate
-
Eubacterium barkeri
iodoacetamide alkylated enzyme Eubacterium barkeri
Itaconate
-
Eubacterium barkeri
mesaconate
-
Eubacterium barkeri
p-chloromercuriphenylsulfonic acid
-
Eubacterium barkeri
succinate
-
Eubacterium barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1
-
2-Methyleneglutarate
-
Eubacterium barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
sucrose density gradient centrifugation Eubacterium barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Eubacterium barkeri enzyme is induced by growth on nicotinic acid and is not detectable in cells grown on glucose ?
-
?

Organism

Organism UniProt Comment Textmining
Eubacterium barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Eubacterium barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Eubacterium barkeri

Storage Stability

Storage Stability Organism
-80°C, protein concentration 1 mg/ml, 38% loss of activity after 6 days Eubacterium barkeri
0°C, protein concentration 1 mg/ml, complete loss of activity after 3 days Eubacterium barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Methyleneglutarate r Eubacterium barkeri 2-Methylene-3-methylsuccinate i.e methylitaconate ?
2-Methyleneglutarate the equilibrium favours the formation of 2-methyleneglutarate Eubacterium barkeri 2-Methylene-3-methylsuccinate i.e methylitaconate ?
additional information enzyme is induced by growth on nicotinic acid and is not detectable in cells grown on glucose Eubacterium barkeri ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 sharp decrease in activity below pH 7.5 and above pH 8 Eubacterium barkeri

Cofactor

Cofactor Comment Organism Structure
cobamide required Eubacterium barkeri
cobamide e.g. alpha-(5,6-dimethylbenzimidazolyl)cobamide coenzyme, Km: 0.000073 mM, alpha-(benzimidazolyl)-cobamide coenzyme, Km: 0.0003 mM, or alpha-(adenyl)-cobamide coenzyme, Km: 0.00125 mM. The most effective coenzyme is alpha(5,6-dimethylbenzimidazolyl)-cobamide coenzyme Eubacterium barkeri