Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl groups | the enzyme is a sulfhydryl protein | Eubacterium barkeri |
General Stability | Organism |
---|---|
enzyme rapidly decays, when stored at low protein concentrations, 1 mg/ml. Complete loss of activity after 3 days at 0°C and 38% loss of activity after 6 days at -80°C | Eubacterium barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Methyl-1,2-cis-cyclopropanedicarboxylate | - |
Eubacterium barkeri | |
1-Methyl-1,2-trans-cyclopropanedicarboxylate | - |
Eubacterium barkeri | |
glutaconate | - |
Eubacterium barkeri | |
iodoacetamide | alkylated enzyme | Eubacterium barkeri | |
Itaconate | - |
Eubacterium barkeri | |
mesaconate | - |
Eubacterium barkeri | |
p-chloromercuriphenylsulfonic acid | - |
Eubacterium barkeri | |
succinate | - |
Eubacterium barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
2-Methyleneglutarate | - |
Eubacterium barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
sucrose density gradient centrifugation | Eubacterium barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Eubacterium barkeri | enzyme is induced by growth on nicotinic acid and is not detectable in cells grown on glucose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Eubacterium barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Eubacterium barkeri |
Storage Stability | Organism |
---|---|
-80°C, protein concentration 1 mg/ml, 38% loss of activity after 6 days | Eubacterium barkeri |
0°C, protein concentration 1 mg/ml, complete loss of activity after 3 days | Eubacterium barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Methyleneglutarate | r | Eubacterium barkeri | 2-Methylene-3-methylsuccinate | i.e methylitaconate | ? | |
2-Methyleneglutarate | the equilibrium favours the formation of 2-methyleneglutarate | Eubacterium barkeri | 2-Methylene-3-methylsuccinate | i.e methylitaconate | ? | |
additional information | enzyme is induced by growth on nicotinic acid and is not detectable in cells grown on glucose | Eubacterium barkeri | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | sharp decrease in activity below pH 7.5 and above pH 8 | Eubacterium barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cobamide | required | Eubacterium barkeri | |
cobamide | e.g. alpha-(5,6-dimethylbenzimidazolyl)cobamide coenzyme, Km: 0.000073 mM, alpha-(benzimidazolyl)-cobamide coenzyme, Km: 0.0003 mM, or alpha-(adenyl)-cobamide coenzyme, Km: 0.00125 mM. The most effective coenzyme is alpha(5,6-dimethylbenzimidazolyl)-cobamide coenzyme | Eubacterium barkeri |