Any feedback?
Literature summary for 5.4.99.39 extracted from
- Purification, kinetics, inhibitors and CD for recombinant beta-amyrin synthase from Euphorbia tirucalli L and functional analysis of the DCTA motif, which is highly conserved among oxidosqualene cyclases (2013), FEBS J., 280, 1267-1280.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | at 0.05% highest activity of the enzyme | Euphorbia tirucalli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a polyhistidine-tagged protein in Saccharomyces cerevisiae GIL77, which lacks the lanosterol synthase gene. Expression yield: 5-7 mg | Euphorbia tirucalli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C486A | mutant shows 46% activity compared to wild-type (100%), Km similar to wild-type, kcat decreased | Euphorbia tirucalli |
| C564A | mutant shows 1.6% activity compared to wild-type (100%), Km increased compared to wild-type, kcat highly decreased | Euphorbia tirucalli |
| D485E | mutant shows no activity | Euphorbia tirucalli |
| D485N | mutant shows no activity | Euphorbia tirucalli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (4-bromophenyl)[2-fluoro-4-([6-[methyl(prop-2-en-1-yl)amino]hexyl]oxy)phenyl]methanone | - |
Euphorbia tirucalli | |
| Iminosqualene | - |
Euphorbia tirucalli | |
| N,N-diethyl-2-[[(3beta)-17-methylideneandrost-5-en-3-yl]oxy]ethanamine | - |
Euphorbia tirucalli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0338 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, wild-type | Euphorbia tirucalli | |
| 0.0348 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C486A | Euphorbia tirucalli | |
| 0.0511 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C564A | Euphorbia tirucalli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
SDS-PAGE | Euphorbia tirucalli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euphorbia tirucalli | Q401R6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Euphorbia tirucalli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.352 | - |
pH 7, 30°C | Euphorbia tirucalli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-2,3-oxidosqualene | - |
Euphorbia tirucalli | beta-amyrin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Euphorbia tirucalli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amyrin synthase | - |
Euphorbia tirucalli |
| EtAS | - |
Euphorbia tirucalli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Euphorbia tirucalli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000128 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C564A | Euphorbia tirucalli | |
| 0.34 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C486A | Euphorbia tirucalli | |
| 0.77 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, wild-type | Euphorbia tirucalli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Euphorbia tirucalli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000698 | - |
(4-bromophenyl)[2-fluoro-4-([6-[methyl(prop-2-en-1-yl)amino]hexyl]oxy)phenyl]methanone | pH 7, 30°C | Euphorbia tirucalli | |
| 0.0000134 | - |
Iminosqualene | pH 7, 30°C | Euphorbia tirucalli | |
| 0.000102 | - |
N,N-diethyl-2-[[(3beta)-17-methylideneandrost-5-en-3-yl]oxy]ethanamine | pH 7, 30°C | Euphorbia tirucalli | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000107 | - |
pH 7, 30°C | Euphorbia tirucalli | (4-bromophenyl)[2-fluoro-4-([6-[methyl(prop-2-en-1-yl)amino]hexyl]oxy)phenyl]methanone | |
| 0.0000309 | - |
pH 7, 30°C | Euphorbia tirucalli | Iminosqualene | |
| 0.000142 | - |
pH 7, 30°C | Euphorbia tirucalli | N,N-diethyl-2-[[(3beta)-17-methylideneandrost-5-en-3-yl]oxy]ethanamine | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000025 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C564A | Euphorbia tirucalli | |
| 9.8 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, mutant C486A | Euphorbia tirucalli | |
| 22.83 | - |
(S)-2,3-oxidosqualene | pH 7, 30°C, wild-type | Euphorbia tirucalli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
