Literature summary for 5.4.99.3 extracted from

  • Allaudeen, H.S.; Ramakrishnan, T.
    Biosynthesis of isoleucine and valine in Mycobacterium tuberculosis H37 Rv (1968), Arch. Biochem. Biophys., 125, 199-209.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid required, D-ascorbate shows 80% of L-ascorbate in enhancing the reaction, cannot be replaced by other reducing agents Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
2-acetolactate
-
Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Acetolactate Mycobacterium tuberculosis enzyme of Ile-Val biosynthesis ?
-
?
2-Acetolactate Mycobacterium tuberculosis H37Rv enzyme of Ile-Val biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
H37Rv
-
Mycobacterium tuberculosis H37Rv
-
H37Rv
-

Storage Stability

Storage Stability Organism
90% loss of activity after 2 days, even when kept under frozen state Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Acetolactate
-
Mycobacterium tuberculosis 3-Hydroxy-3-methyl-2-oxobutanoate
-
?
2-Acetolactate
-
Mycobacterium tuberculosis H37Rv 3-Hydroxy-3-methyl-2-oxobutanoate
-
?
2-aceto-2-hydroxybutanoate
-
Mycobacterium tuberculosis 3-hydroxy-3-methyl-2-oxopentanoate
-
?
2-aceto-2-hydroxybutanoate
-
Mycobacterium tuberculosis H37Rv 3-hydroxy-3-methyl-2-oxopentanoate
-
?
2-Acetolactate enzyme of Ile-Val biosynthesis Mycobacterium tuberculosis ?
-
?
2-Acetolactate enzyme of Ile-Val biosynthesis Mycobacterium tuberculosis H37Rv ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Mycobacterium tuberculosis