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Literature summary for 5.4.99.28 extracted from

  • Wrzesinski, J.; Nurse, K.; Bakin, A.; Lane, B.G.; Ofengand, J.
    A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe) (1995), RNA, 1, 437-448.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24432
-
 x * 24432, calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA uridine32 Escherichia coli
-
 tRNA pseudouridine32
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AA37
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine32
-
 Escherichia coli tRNA pseudouridine32
-
 ?
tRNA uridine32 substrate tRNAPhe. The enzyme is also responsible for the formation of pseudouridine746 in 23S rRNA Escherichia coli tRNA pseudouridine32
-
 ?

Subunits

Subunits Comment Organism
? x * 24432, calculated from sequence Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli