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Literature summary for 5.4.99.27 extracted from

  • Guzzi, N.; Ciesla, M.; Ngoc, P.C.T.; Lang, S.; Arora, S.; Dimitriou, M.; Pimkova, K.; Sommarin, M.N.E.; Munita, R.; Lubas, M.; Lim, Y.; Okuyama, K.; Soneji, S.; Karlsson, G.; Hansson, J.; Joensson, G.; Lund, A.H.; Sigvardsson, M.; Hellstroem-Lindberg, E.; Hsieh, A.C.; Bellodi, C.
    Pseudouridylation of tRNA-derived fragments steers translational control in stem cells (2018), Cell, 173, 1204-1216 .
    View publication on PubMed
Search for more literature for 5.4.99.27

Protein Variants

Protein Variants Comment Organism
additional information generation of PUS7-knockout human stem cells, hESCs, using CRISPR/Cas9 technique. There are no differences in cell proliferation, viability, and expression of multiple pluripotency, and germ layer specific markers between wild-type and PUS7-KO cells maintained in culture over long periods of time without loss of self-renewal, but a significant increase in PUS7-KO cell size compared to controls is observed. Knockout of PUS7 in HEK-293T cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96PZ0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryonic stem cell
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 Homo sapiens
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hematopoietic stem cell
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 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine13
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 Homo sapiens tRNA pseudouridine13
-
 ?

Synonyms

Synonyms Comment Organism
PSI
-
 Homo sapiens
PUS7
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction PUS7 inactivation in embryonic stem cells impairs tRNA-derived small fragments (tRFs)-mediated translation regulation, leading to increased protein biosynthesis and defective germ layer specification. Dysregulation of this posttranscriptional regulatory circuitry impairs hematopoietic stem cell commitment and is common to aggressive subtypes of human myelodysplastic syndromes, overview. PUS7 loss impacts early embryogenesis and mesoderm specification Homo sapiens
physiological function pseudouridylation (Psi) is the most abundant and widespread type of RNA epigenetic modification in living organisms. Psi-driven posttranscriptional program steers translation control to impact stem cell commitment during early embryogenes. Mechanistically, the Psi writer PUS7 modifies and activates a network of tRNA-derived small fragments (tRFs) targeting the translation initiation complex. Critical function of Psi in directing translation control in stem cells with important implications for development and disease. PUS7 Psi synthase activity impacts stem cell size and protein biosynthesis. PUS7 binds specific tRNA isoacceptors in human stem cells, hESCs. PUS7-mediated Psi directs tRNA-derived small RNAs to inhibit translation. Psi is critically required for 5'tRF-dependent translational repression in vivo. Pseudouridylated mTOG targets the translation initiation complex. PUS7-mediated Psi critically governs hematopoietic stem cell function Homo sapiens