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Literature summary for 5.4.99.27 extracted from

  • Schwartz, S.; Bernstein, D.A.; Mumbach, M.R.; Jovanovic, M.; Herbst, R.H.; Leon-Ricardo, B.X.; Engreitz, J.M.; Guttman, M.; Satija, R.; Lander, E.S.; Fink, G.; Regev, A.
    Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA (2014), Cell, 159, 148-162 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
transcriptome-wide quantitative mapping of Psi Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus7-dependent sites at position 13 of glutamate tRNA. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions ?
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 ?
tRNA uridine13 Saccharomyces cerevisiae
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 tRNA pseudouridine13
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08647
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus7-dependent sites at position 13 of glutamate tRNA. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions Saccharomyces cerevisiae ?
-
 ?
tRNA uridine13
-
 Saccharomyces cerevisiae tRNA pseudouridine13
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 ?

Synonyms

Synonyms Comment Organism
PUS7
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 Saccharomyces cerevisiae

General Information

General Information Comment Organism
additional information transcriptome-wide quantitative mapping of Psi, method development using Psi-seq, relying on the unique stability of N3-[N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethylcarbodiimide-Psi] (N3-CMC-Psi) to alkaline hydrolysis, and the ability of N3-CMC-Psi to terminate reverse transcription. For rRNA, 24/24 Cbf5-dependent sites are known targets of pseudouridylation, 8/9 associations between snoRNAs and rRNA sites are known, as is the identified Pus5-mediated Psi site in mitochondrial 21S rRNA. One snR3-dependent site at position 2140 on 25S rRNA is not specificated, detailed overview. Cbf5-dependent-Psi sites in mRNAs and snoRNAs are likely snoRNA-guided Saccharomyces cerevisiae
physiological function the most abundant RNA modification is pseudouridine (Psi), Psi is ubiquitous in diverse RNAs, and dynamic in mRNA. Pseudouridylation profiles and mechanisms. Pus7-dependent induction of pseudouridylation during heat shock Saccharomyces cerevisiae