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Literature summary for 5.4.99.21 extracted from

  • Alian, A.; DeGiovanni, A.; Griner, S.L.; Finer-Moore, J.S.; Stroud, R.M.
    Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome (2009), J. Mol. Biol., 388, 785-800.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method, X-ray crystal structure of RluF bound to the isolated stem–loop, in which uridine2604 is substituted by 5-fluorouridine to prevent catalytic turnover Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P32684
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
23S rRNA uridine2604 = 23S rRNA pseudouridine2604 the substrate of RluF undergoes radical structural rearrangements upon binding. The RluF target base lies in an RNA stem rather than in a flexible loop and the substrate conformational change requires disruption of multiple base pairs. This energetically costly process is driven by interactions of the RNA with the protein, a mechanism of RluF is proposed Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23S rRNA uridine2604 RluF does not modify U2604 in rRNA mutant stem-loops that lack the A2602 bulge and shows dramatically higher activity for a stem-loop with a mutation designed to facilitate A2602 refolding into the stem with concomitant RNA strand translation. The substrate of RluF undergoes radical structural rearrangements upon binding. The RluF target base lies in an RNA stem rather than in a flexible loop and the substrate conformational change requires disruption of multiple base pairs. This energetically costly process is driven by interactions of the RNA with the protein Escherichia coli 23S rRNA pseudouridine2604
-
 ?

Synonyms

Synonyms Comment Organism
RluF
-
 Escherichia coli