Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method, domain architecture and crystal structure of the catalytic domain of RluF at 2.6 A resolution. Limited proteolysis, mass spectrometry and N-terminal sequencing indicate that RluF has a distinct domain architecture, with the catalytic domain flanked at the N and C termini by additional domains connected to it by flexible linkers. The structure of the catalytic domain of RluF is similar to those of RsuA and TruB. Structural comparison of RluF with its closest structural homologues, RsuA and TruB, suggests possible functional roles for the N-terminal and C-terminal domains of RluF | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P32684 | - |
- |
Purification (Comment) | Organism |
---|---|
full-length RluF and N-terminal, 65-residue-truncated RulF (DELTARulF) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
23S rRNA uridine2604 | - |
Escherichia coli | 23S rRNA pseudouridine2604 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RluF | - |
Escherichia coli |