Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Protein Variants | Comment | Organism |
---|---|---|
Y243A | 40000fold decrease in catalytic efficiency, twofold decrease of cob(II)alamin concentration under steady state turnover conditions, no loss of stereochemical preference for substrate | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
allylmalonyl-CoA | competitive with wild-type, O2-independent suicide inactivation with mutant Y243A via an internal electron transfer from cob(II)alamin to the inhibitor radical | Propionibacterium freudenreichii subsp. shermanii | |
ethylmalonyl-CoA | competitive with wild-type, O2-dependent suicide inactivation with mutant Y243A | Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for mutant Y243A with substrate (R)-2-methylmalonyl-CoA is above 10 mM | Propionibacterium freudenreichii subsp. shermanii | |
0.124 | - |
(R)-2-methylmalonyl-CoA | wild-type, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-methylmalonyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | succinyl-CoA | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
(R)-2-methylmalonyl-CoA | mutant Y243A, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
158 | - |
(R)-2-methylmalonyl-CoA | wild-type, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | - |
Propionibacterium freudenreichii subsp. shermanii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
allylmalonyl-CoA | mutant Y243A, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
0.0153 | - |
ethylmalonyl-CoA | mutant Y243A, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
0.425 | - |
allylmalonyl-CoA | wild-type, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
0.467 | - |
ethylmalonyl-CoA | wild-type, pH 7.0, 37°C | Propionibacterium freudenreichii subsp. shermanii |