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Literature summary for 5.4.99.2 extracted from

  • Padovani, D.; Banerjee, R.
    Alternative pathways for radical dissipation in an active site mutant of B12-dependent methylmalonyl-CoA mutase (2006), Biochemistry, 45, 2951-2959.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Propionibacterium freudenreichii subsp. shermanii

Protein Variants

Protein Variants Comment Organism
Y243A 40000fold decrease in catalytic efficiency, twofold decrease of cob(II)alamin concentration under steady state turnover conditions, no loss of stereochemical preference for substrate Propionibacterium freudenreichii subsp. shermanii

Inhibitors

Inhibitors Comment Organism Structure
allylmalonyl-CoA competitive with wild-type, O2-independent suicide inactivation with mutant Y243A via an internal electron transfer from cob(II)alamin to the inhibitor radical Propionibacterium freudenreichii subsp. shermanii
ethylmalonyl-CoA competitive with wild-type, O2-dependent suicide inactivation with mutant Y243A Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value for mutant Y243A with substrate (R)-2-methylmalonyl-CoA is above 10 mM Propionibacterium freudenreichii subsp. shermanii
0.124
-
(R)-2-methylmalonyl-CoA wild-type, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-methylmalonyl-CoA
-
Propionibacterium freudenreichii subsp. shermanii succinyl-CoA
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
(R)-2-methylmalonyl-CoA mutant Y243A, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii
158
-
(R)-2-methylmalonyl-CoA wild-type, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii

Cofactor

Cofactor Comment Organism Structure
Cobalamin
-
Propionibacterium freudenreichii subsp. shermanii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0109
-
allylmalonyl-CoA mutant Y243A, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii
0.0153
-
ethylmalonyl-CoA mutant Y243A, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii
0.425
-
allylmalonyl-CoA wild-type, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii
0.467
-
ethylmalonyl-CoA wild-type, pH 7.0, 37°C Propionibacterium freudenreichii subsp. shermanii