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Literature summary for 5.4.99.2 extracted from
- Purification and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium meliloti (2003), Arch. Microbiol., 180, 151-154.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Sinorhizobium meliloti |  |
| Li+ | slight inhibition at 3 mM | Sinorhizobium meliloti | |
| Na+ | slight inhibition at 3 mM | Sinorhizobium meliloti | |
| p-chloromercuribenzoate | - |
Sinorhizobium meliloti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00064 | - |
adenosylcobalamin | 37°C, pH 7, in presence of 3 mM NH4+ | Sinorhizobium meliloti | |
| 0.0086 | - |
adenosylcobalamin | 37°C, pH 7, without NH4+ | Sinorhizobium meliloti | |
| 0.11 | - |
(R,S)-methylmalonyl-CoA | 37°C, pH 7, in presence of 3 mM NH4+ | Sinorhizobium meliloti | |
| 0.13 | - |
(R,S)-methylmalonyl-CoA | 37°C, pH 7, without NH4+ | Sinorhizobium meliloti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | stimulates at 3 mM | Sinorhizobium meliloti | |
| Rb+ | stimulates at 3 mM | Sinorhizobium meliloti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
alpha2, 2 * 80000, SDS-PAGE | Sinorhizobium meliloti |
| 165000 | - |
gel filtration | Sinorhizobium meliloti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-methylmalonyl-CoA | Sinorhizobium meliloti | - |
succinyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Sinorhizobium meliloti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.85 | - |
after UNO Q-1 purification | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-methylmalonyl-CoA | - |
Sinorhizobium meliloti | succinyl-CoA | - |
? | |
| methylmalonyl-CoA | - |
Sinorhizobium meliloti | succinyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, 2 * 80000, SDS-PAGE | Sinorhizobium meliloti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCM | - |
Sinorhizobium meliloti |
| methylmalonyl-CoA mutase | - |
Sinorhizobium meliloti |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Sinorhizobium meliloti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Sinorhizobium meliloti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adenosylcobalamin | - |
Sinorhizobium meliloti | |
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