BRENDA - Enzyme Database show
show all sequences of 5.4.99.19

16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases

Conrad, J.; Niu, L.; Rudd, K.; Lane, B.G.; Ofengand, J.; RNA 5, 751-763 (1999)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D102N
mutation at a conserved aspartate inactivates in vivo production of Psi516
Escherichia coli
D102T
mutation at a conserved aspartate inactivates in vivo production of Psi516
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0AA43
-
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D102N
mutation at a conserved aspartate inactivates in vivo production of Psi516
Escherichia coli
D102T
mutation at a conserved aspartate inactivates in vivo production of Psi516
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
General Information
General Information
Commentary
Organism
malfunction
deletion of this gene results in the specific loss of pseudouridine516. No effect on the growth rate of rsuA– MG1655 either in rich or minimal medium at either 24°C, 37°C, or 42°C
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion of this gene results in the specific loss of pseudouridine516. No effect on the growth rate of rsuA– MG1655 either in rich or minimal medium at either 24°C, 37°C, or 42°C
Escherichia coli
Other publictions for EC 5.4.99.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
653325
Sivaraman
Structure of the 16S rRNA pseu ...
Escherichia coli
Nat. Struct. Biol.
9
353-358
2002
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
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-
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-
-
-
-
706732
Conrad
16S ribosomal RNA pseudouridin ...
Escherichia coli
RNA
5
751-763
1999
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
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-
-
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2
-
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1
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1
1
-
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-
702191
Wrzesinski
Purification, cloning, and pro ...
Escherichia coli
Biochemistry
34
8904-8913
1995
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-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
1
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-
-
-
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2
1
1
-
-
-
1
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
1
-
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-
1
-
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-
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