Literature summary for 5.4.99.17 extracted from

Hammer, S.C.; Marjanovic, A.; Dominicus, J.M.; Nestl, B.M.; Hauer, B.
Squalene hopene cyclases are protonases for stereoselective Bronsted acid catalysis (2015), Nat. Chem. Biol., 11, 121-126 .

Search for more literature for 5.4.99.17

Protein Variants

Protein Variants	Comment	Organism
A419F	inactive	Alicyclobacillus acidocaldarius
D376C	inactive	Alicyclobacillus acidocaldarius
F365A	the mutant shows increased activity with geraniol and (S)-6,7-epoxygeraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
F365C	the mutant exhibits 15% conversion of geraniol to gamma-cyclogeraniol	Alicyclobacillus acidocaldarius
F365C	the mutant shows increased activity with geraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
F365G	the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
F365W	inactive	Alicyclobacillus acidocaldarius
F601A	the mutant shows about wild type activity	Alicyclobacillus acidocaldarius
F601W	the mutant shows about wild type activity	Alicyclobacillus acidocaldarius
G600F	the mutant exhibits 68% conversion of geraniol to cyclogeraniol hydrate	Alicyclobacillus acidocaldarius
I261A	the mutant exhibits 11% conversion of citronellal to (-)-iso-isopulegol. The mutant shows increased activity with geraniol and (S)-6,7-epoxygeraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
I261W	the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
L36A	the mutant shows increased activity with (S)-6,7-epoxygeraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
L36W	inactive	Alicyclobacillus acidocaldarius
S307A	the mutant shows increased activity with (S)-6,7-epoxygeraniol and no activity with geraniol compared to the wild type enzyme	Alicyclobacillus acidocaldarius
S307F	inactive	Alicyclobacillus acidocaldarius
V448F	inactive	Alicyclobacillus acidocaldarius
W169A	the mutant shows about wild type activity	Alicyclobacillus acidocaldarius
W312A	inactive	Alicyclobacillus acidocaldarius
W489A	inactive	Alicyclobacillus acidocaldarius
Y420A	inactive	Alicyclobacillus acidocaldarius
Y420C	the mutant shows about wild type activity	Alicyclobacillus acidocaldarius
Y420W	the mutant exhibits 54% conversion of (S)-6,7-epoxygeraniol to (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol	Alicyclobacillus acidocaldarius
Y420W/G600F	the mutant exhibits 78% conversion of (S)-6,7-epoxygeraniol to (1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol	Alicyclobacillus acidocaldarius
Y609W	inactive	Alicyclobacillus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
squalene	Alicyclobacillus acidocaldarius	-	hop-22(29)-ene	-	?
squalene + H2O	Alicyclobacillus acidocaldarius	-	hopan-22-ol	-	?

Organism

Organism	UniProt	Comment	Textmining
Alicyclobacillus acidocaldarius	P33247	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(S)-6,7-epoxygeraniol + H2O	0.57% conversion with high enzyme concentrations	Alicyclobacillus acidocaldarius	(1S,3R)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol	-	?
(S)-citronellal + H2O	0.59% conversion with high enzyme concentrations	Alicyclobacillus acidocaldarius	(-)-iso-isopulegol	-	?
geraniol + H2O	0.4% conversion with high enzyme concentrations	Alicyclobacillus acidocaldarius	gamma-cyclogeraniol + cyclogeraniol hydrate	-	?
squalene	-	Alicyclobacillus acidocaldarius	hop-22(29)-ene	-	?
squalene + H2O	-	Alicyclobacillus acidocaldarius	hopan-22-ol	-	?

Synonyms

Synonyms	Comment	Organism
SHC	-	Alicyclobacillus acidocaldarius
squalene hopene cyclase	-	Alicyclobacillus acidocaldarius