Cloned (Comment) | Organism |
---|---|
gene treSB, DNA and amino acid sequence determination and analysis, recombinant expression in Bacillus subtilis strain W800N, recombinant expression of His6-tagged enzyme in Escherichia coli strain DH5alpha | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pseudomonas putida | |
Zn2+ | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Pseudomonas putida | |
Mg2+ | activates | Pseudomonas putida | |
Na+ | activates | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | Pseudomonas putida | - |
alpha,alpha-trehalose | - |
r | |
maltose | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | - |
alpha,alpha-trehalose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88FN0 | - |
- |
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | Q88FN0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme by nickel affinity chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
Pseudomonas putida | alpha,alpha-trehalose | - |
r | |
maltose | - |
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | alpha,alpha-trehalose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Trehalose synthase | - |
Pseudomonas putida |
TreS | - |
Pseudomonas putida |
treSB | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
recombinant His6-tagged enzyme | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant His6-tagged enzyme | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | purified recombinant His6-tagged enzyme, stable at | Pseudomonas putida |