Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermomonospora curvata |
Protein Variants | Comment | Organism |
---|---|---|
additional information | SpyTag and SpyCatcher can spontaneously and rapidly conjugate to form an irreversible and stable covalent bond. The trehalose synthase (TreS) from Thermomonospora curvata is successfully cyclized after the fusion of a SpyTag to its C-terminus and SpyCatcher to the N-terminus | Thermomonospora curvata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | Thermomonospora curvata | - |
alpha,alpha-trehalose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomonospora curvata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Thermomonospora curvata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
Thermomonospora curvata | alpha,alpha-trehalose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Trehalose synthase | - |
Thermomonospora curvata |
TreS | - |
Thermomonospora curvata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermomonospora curvata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 70 | activity range, profile overview | Thermomonospora curvata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | recombinant cyclized TreS retains more than 85% of its activity at temperatures ranging from 40°C to 50°C, while the wild type kept only 60% of its activity under the same conditions | Thermomonospora curvata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Thermomonospora curvata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | activity range, profile overview | Thermomonospora curvata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | recombinant cyclized TreS retains more than 85% of its activity at a pH range of 8.0 to 10.0, while the wild type kept only 80% of its activity under the same conditions. Wild-type and linear TreS lost about 20% and 15% of their original activities after incubation in this pH range, respectively | Thermomonospora curvata |