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Literature summary for 5.4.99.16 extracted from

  • Xu, C.; Xu, Q.; Huang, H.; Jiang, L.
    Enhancing the stability of trehalose synthase via SpyTag/SpyCatcher cyclization to improve its performance in industrial biocatalysts (2018), Biosci. Biotechnol. Biochem., 82, 1473-1479 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Thermomonospora curvata

Protein Variants

Protein Variants Comment Organism
additional information SpyTag and SpyCatcher can spontaneously and rapidly conjugate to form an irreversible and stable covalent bond. The trehalose synthase (TreS) from Thermomonospora curvata is successfully cyclized after the fusion of a SpyTag to its C-terminus and SpyCatcher to the N-terminus Thermomonospora curvata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maltose Thermomonospora curvata
-
alpha,alpha-trehalose
-
r

Organism

Organism UniProt Comment Textmining
Thermomonospora curvata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Thermomonospora curvata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
Thermomonospora curvata alpha,alpha-trehalose
-
r

Synonyms

Synonyms Comment Organism
Trehalose synthase
-
Thermomonospora curvata
TreS
-
Thermomonospora curvata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Thermomonospora curvata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 70 activity range, profile overview Thermomonospora curvata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 recombinant cyclized TreS retains more than 85% of its activity at temperatures ranging from 40°C to 50°C, while the wild type kept only 60% of its activity under the same conditions Thermomonospora curvata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Thermomonospora curvata

pH Range

pH Minimum pH Maximum Comment Organism
3 11 activity range, profile overview Thermomonospora curvata

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 recombinant cyclized TreS retains more than 85% of its activity at a pH range of 8.0 to 10.0, while the wild type kept only 80% of its activity under the same conditions. Wild-type and linear TreS lost about 20% and 15% of their original activities after incubation in this pH range, respectively Thermomonospora curvata