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Literature summary for 5.4.99.16 extracted from
- Preparation of cross-linked enzyme aggregates of trehalose synthase via co-aggregation with polyethyleneimine (2014), Appl. Biochem. Biotechnol., 174, 2067-2078 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene treS, recombinant expression of His-tagged enzyme in Escherichia coli | Meiothermus ruber |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | application of TreS is quite limited, due to the difficulty in recovering the enzyme from reaction mixtures and its intolerance and instability under extreme circumstances. In order to overcome these limitations, immobilization of TreS is developed using cross-linked enzyme aggregates (CLEAs), a kind of carrier-free immobilization technology. Immobilization of purified His-tagged enzyme on CLEAs-PEI-PEG particles, preparation and microstructures of CLEAs-PEI-PEG and CLEAs-PEI. Method optimization and evaluation, overview. 25% PEG as aggregates precipitant gives maximum activity recovery (65.4%), usage of glutaraldehyde is best in a ratio of 1:0.5 | Meiothermus ruber |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | Meiothermus ruber | - |
alpha,alpha-trehalose | - |
r |  |
| maltose | Meiothermus ruber CBS-01 | - |
alpha,alpha-trehalose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meiothermus ruber | B1PK99 | isolated from geothermal water | - |
| Meiothermus ruber CBS-01 | B1PK99 | isolated from geothermal water | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Meiothermus ruber |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Meiothermus ruber | alpha,alpha-trehalose | - |
r | |
| maltose | an enzymatic intramolecular rearrangement reaction | Meiothermus ruber | alpha,alpha-trehalose | - |
r | |
| maltose | - |
Meiothermus ruber CBS-01 | alpha,alpha-trehalose | - |
r | |
| maltose | an enzymatic intramolecular rearrangement reaction | Meiothermus ruber CBS-01 | alpha,alpha-trehalose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Meiothermus ruber |
| TreS | - |
Meiothermus ruber |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
free recombinant enzyme | Meiothermus ruber |
| 60 | - |
immobilized recombinant enzyme | Meiothermus ruber |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | recombinant free enzyme, active at, inactivation above 70°C, profile overview | Meiothermus ruber |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant | Meiothermus ruber |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | recombinant free enzyme, activity range, profile overview | Meiothermus ruber |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | recombinant free enzyme, 70°C, 2 h, over 80% activity remaning within this range | Meiothermus ruber |
| 5 | 10 | recombinant immobilized enzyme, 70°C, 2 h, over 80% activity remaning within this range | Meiothermus ruber |
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Release 2023.1 (January 2023)
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