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Literature summary for 5.4.99.16 extracted from
- Identification and characterization of a novel trehalose synthase gene derived from saline-alkali soil metagenomes (2013), PLoS ONE, 8, e77437.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is a good candidate in the large-scale production of trehalose from maltose | uncultured bacterium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene treS, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic tree, recombinant overexression in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain D5alpha | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | slight inhibition | uncultured bacterium |  |
| Hg2+ | - |
uncultured bacterium | |
| additional information | no or poor inhibition by MgCl2, MnCl, BaCl2, CaCl2, SrCl2, NiCl2, CoCl2Al2(SO4), FeSO4, DTT, and EDTA | uncultured bacterium | |
| Pb2+ | slight inhibition | uncultured bacterium | |
| SDS | - |
uncultured bacterium | |
| Tris | - |
uncultured bacterium | |
| Zn2+ | - |
uncultured bacterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63300 | - |
x * 63300, about, sequence calculation | uncultured bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | uncultured bacterium | - |
alpha,alpha-trehalose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
isolated from saline-alkali soil, gene treS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) | uncultured bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 133.5 | - |
purified recombinant enzyme, pH 9.0, 45°C | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
uncultured bacterium | alpha,alpha-trehalose | - |
r | |
| maltose | the recombinant enzyme has a 4.1fold higher catalytic efficientcy for maltose than for trehalose | uncultured bacterium | alpha,alpha-trehalose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63300, about, sequence calculation | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
uncultured bacterium |
| TreS | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 55 | over 30% of maximal activity within this range | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | over 50% of maximal activity within this range | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 13 | uncultured bacterium |
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Release 2023.1 (January 2023)
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