Cloned (Comment) | Organism |
---|---|
gene treS, sequence comparison, expression of His6-tagged wild-type enzyme and chimeric mutant in Escherichia coli strain Rosetta-gami (DE3), subcloning in Escherichia coli strain DH5alpha | Thermus thermophilus |
gene treS, sequence comparison, expression of His6-tagged wild-type enzyme and the chimeric mutant in Escherichia coli strain Rosetta-gami (DE3), subcloning in Escherichia coli strain DH5alpha | Meiothermus ruber |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric enzyme mutant consisting of the C-terminus from Meiothermus ruber trehalose synthase and the N-terminus from Thermus thermophilus trehalose synthase, TSMrTt , and a second with N-terminus from Meiothermus ruber trehalose synthase and C-terminus from Thermus thermophilus trehalose synthase, TSTtMr | Meiothermus ruber |
additional information | construction of a chimeric enzyme mutant consisting of the C-terminus from Meiothermus ruber trehalose synthase and the N-terminus from Thermus thermophilus trehalose synthase, TSMrTt , and a second with N-terminus from Meiothermus ruber trehalose synthase and C-terminus from Thermus thermophilus trehalose synthase, TSTtMr | Thermus thermophilus |
R392A | site-directed mutagenesis, the mutant shows a sharp decrease in activity compared to the wild-type enzyme | Meiothermus ruber |
R392F | site-directed mutagenesis, the mutation leads to a complete loss in activity | Meiothermus ruber |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
57.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
57.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
69.6 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
69.6 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
79.2 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
79.2 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
82.2 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
97.4 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
98.9 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
126 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
154 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
154 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | Meiothermus ruber | - |
alpha,alpha-trehalose | - |
r | |
maltose | Thermus thermophilus | - |
alpha,alpha-trehalose | - |
r | |
maltose | Meiothermus ruber CBS-01 | - |
alpha,alpha-trehalose | - |
r | |
maltose | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
alpha,alpha-trehalose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meiothermus ruber | B1PK99 | gene treS | - |
Meiothermus ruber CBS-01 | B1PK99 | gene treS | - |
Thermus thermophilus | Q5SL15 | gene treS | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SL15 | gene treS | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and chimeric mutant enzymes from Escherichia coli strain Rosetta-gami (DE3) by nickel affinity chromatography | Meiothermus ruber |
recombinant His6-tagged wild-type and chimeric mutant enzymes from Escherichia coli strain Rosetta-gami (DE3) by nickel affinity chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose | - |
Meiothermus ruber | maltose | - |
r | |
alpha,alpha-trehalose | - |
Thermus thermophilus | maltose | - |
? | |
alpha,alpha-trehalose | - |
Meiothermus ruber CBS-01 | maltose | - |
r | |
alpha,alpha-trehalose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | maltose | - |
? | |
maltose | - |
Meiothermus ruber | alpha,alpha-trehalose | - |
r | |
maltose | - |
Thermus thermophilus | alpha,alpha-trehalose | - |
r | |
maltose | - |
Meiothermus ruber CBS-01 | alpha,alpha-trehalose | - |
r | |
maltose | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | alpha,alpha-trehalose | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | the isolated N-terminal domain from Meiothermus ruber is not active. The secondary structure of the isolated N-terminal domain undergoes a greater change than that of the isolated C-terminus, three-dimensional structure analysis and modeling, overview | Thermus thermophilus |
More | the isolated N-terminal domain from Meiothermus ruber is not active. The secondary structure of the isolated N-terminal domain undergoes a greater change than that of the isolated C-terminus, three-dimensional structure enzyme structure analysis and molecular modeling, overview | Meiothermus ruber |
Synonyms | Comment | Organism |
---|---|---|
Trehalose synthase | - |
Meiothermus ruber |
Trehalose synthase | - |
Thermus thermophilus |
TreS | - |
Meiothermus ruber |
TreS | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant wild-type enzyme and mutant TSMrTt | Meiothermus ruber |
60 | - |
recombinant wild-type enzyme and mutant TSTtMr | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-lives of purified wild-type enzyme: 48.5 min, purified mutant TSMrTt : 47.6 min, and purified mutant TSTtMr: over 480 min, mutant R392A: 42.2 min | Meiothermus ruber |
70 | - |
half-lives of purified wild-type enzyme: over 480 min, purified mutant TSMrTt : 47.6 min, and purified mutant TSTtMr: over 480 min | Thermus thermophilus |
75 | - |
half-lives of purified wild-type enzyme: 15.8 min, purified mutant TSMrTt : 17.6 min, and purified mutant TSTtMr: over 480 min, mutant R392A: 12.5 min | Meiothermus ruber |
75 | - |
half-lives of purified wild-type enzyme: 475 min, purified mutant TSMrTt : 17.6 min, and purified mutant TSTtMr: over 480 min | Thermus thermophilus |
80 | - |
half-lives of purified wild-type enzyme: 233 min, purified mutant TSMrTt : below 5 min, and purified mutant TSTtMr: 27.6 min | Thermus thermophilus |
80 | - |
half-lives of purified wild-type enzyme: below 5 min, purified mutant TSMrTt : below 5 min, and purified mutant TSTtMr: 27.6 min, mutant R392A: below 5 min | Meiothermus ruber |
100 | - |
purified enzyme, 10 min, inactivation | Meiothermus ruber |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
68.9 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
110 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
115 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
115 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
147 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
152 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
152 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
202 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
227 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Meiothermus ruber |
6.5 | - |
assay at | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure analysis and molecular modeling, overview | Meiothermus ruber |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.697 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
0.871 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
0.871 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
1.16 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Meiothermus ruber | |
1.32 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
1.32 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus | |
1.33 | - |
alpha,alpha-trehalose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
1.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Meiothermus ruber | |
1.5 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSMrTt | Thermus thermophilus | |
2.51 | - |
maltose | pH 6.5, 30°C, recombinant wild-type enzyme | Thermus thermophilus | |
2.7 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Meiothermus ruber | |
2.7 | - |
maltose | pH 6.5, 30°C, recombinant mutant TSTtMr | Thermus thermophilus |