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Literature summary for 5.4.99.16 extracted from

  • Chou, H.; Chang, S.; Lee, G.; Chen, Y.; Yeh, T.; Akoh, C.; Shaw, J.
    Site-directed mutagenesis improves the thermostability of a recombinant Picrophilus torridus trehalose synthase and efficiency for the production of trehalose from sweet potato starch (2010), Food Chem., 119, 1017-1022.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A255P 2.0% activity compared to the wild type enzyme Picrophilus torridus
D411P 3.2% activity compared to the wild type enzyme Picrophilus torridus
D41P 99.6% activity compared to the wild type enzyme Picrophilus torridus
E469P 50% activity compared to the wild type enzyme Picrophilus torridus
I121P 21.3% activity compared to the wild type enzyme Picrophilus torridus
I385P 5.1% activity compared to the wild type enzyme Picrophilus torridus
K332P 96.7% activity compared to the wild type enzyme Picrophilus torridus
N503P the mutant shows about 39% higher relative activity than that of the wild type at 65°C for 120 min. The trehalose yield of the mutant is 1.3fold higher than that of the wild type with sweet potato starch as substrate at 50°C for 4 h Picrophilus torridus
R523P 90.8% activity compared to the wild type enzyme Picrophilus torridus
S439P 20.8% activity compared to the wild type enzyme Picrophilus torridus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Picrophilus torridus

Organism

Organism UniProt Comment Textmining
Picrophilus torridus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Chelating HP column chromatography Picrophilus torridus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
cell-free extract, at pH 6.0 and 45°C for 25 min using maltose as substrate Picrophilus torridus
0.25
-
after 5.3fold purification, at pH 6.0 and 45°C for 25 min using maltose as substrate Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
Picrophilus torridus alpha,alpha-trehalose
-
?
starch
-
Picrophilus torridus ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Picrophilus torridus

Synonyms

Synonyms Comment Organism
PTTS
-
Picrophilus torridus
Trehalose synthase
-
Picrophilus torridus
TSase
-
Picrophilus torridus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the wild type enzyme retains 40% of activity after 120 min at 65°C Picrophilus torridus